Kalafatas P, Voulgaris E, Vorias N, Kotsifopoulos P
Acta Haematol. 1977;58(3):181-8. doi: 10.1159/000207826.
The susceptibility to autoxidation of red cell lipids was studied before and after transformation of normal red cells to PNH-like erythrocytes. The transformation was effected by treatment of the red cells with the sulfydryl compounds D-penicillamine (DP) and N-acetyl-L-cysteine (NAC). The autoxidation was induced by incubating the cells with H2O2 and was estimated by measuring the generated malonyl dialdehyde. The susceptibility to autoxidation was significantly higher in DP-treated cells, while the opposite was true for NAC-treated cells. However, both DP- and NAC-treated cells showed a similar sensitivity to lysis by acid serum and about the same degree of acetylcholinesterase (AChE) activity decrease, thus indicating that the susceptibility to autoxidation of lipids is not involved in the determination of complement sensitivity or in the AChE activity decrease of the sulfydryl-treated cells. Finally, since, as evidenced from most of the reported cases in the literature, increased susceptibility to autoxidation is a feature of PNH cells, it seems reasonable to suggest that DP-treated cells should be used in preference to NAC-treated cells as a laboratory substitute for PNH cells.
在正常红细胞转变为类阵发性睡眠性血红蛋白尿症（PNH）红细胞之前和之后，研究了红细胞脂质的自氧化敏感性。通过用巯基化合物D-青霉胺（DP）和N-乙酰-L-半胱氨酸（NAC）处理红细胞来实现这种转变。通过将细胞与过氧化氢孵育来诱导自氧化，并通过测量生成的丙二醛来评估。DP处理的细胞对自氧化的敏感性显著更高，而NAC处理的细胞则相反。然而，DP和NAC处理的细胞对酸血清裂解表现出相似的敏感性，并且乙酰胆碱酯酶（AChE）活性降低的程度大致相同，因此表明脂质对自氧化的敏感性与补体敏感性的测定或巯基处理细胞的AChE活性降低无关。最后，由于从文献中报道的大多数病例来看，自氧化敏感性增加是PNH细胞的一个特征，因此似乎有理由建议，作为PNH细胞的实验室替代物，应优先使用DP处理的细胞而非NAC处理的细胞。
