阵发性睡眠性血红蛋白尿（PNH）及类PNH红细胞膜上补体蛋白的检测。
An examination of complement proteins on membranes of paroxysmal nocturnal haemoglobinuria (PNH) and PNH-like red cells.
作者信息
Burapakulsolsri N, Yuthavong Y, Wilairat P
出版信息
Br J Haematol. 1979 Mar;41(3):393-8. doi: 10.1111/j.1365-2141.1979.tb05872.x.
Abstract
Proteins obtained from membranes of PNH erythrocytes and of red cells treated with 2-aminoethylisothiouronium bromide (AET) following lysis in acidified serum (Ham's test) were compared by sodium dodecylsulphate-polyacrylamide gel electrophoresis. The peptide patterns were identical, providing support that AET-treated red cells afford a suitable experimental substitute for PNH red cells in studies involving complement fixation.
摘要
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，比较了酸化血清中溶血后从阵发性睡眠性血红蛋白尿症（PNH）红细胞膜以及用2-氨基乙基异硫脲溴化物（AET）处理的红细胞膜中获得的蛋白质。肽图谱是相同的，这支持了在涉及补体固定的研究中，经AET处理的红细胞可作为PNH红细胞的合适实验替代物。
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