Chen Mei-Na, Ge Yu-Qi, Zhao Yu-Zhu, Lin Zhen-Fu, Li Hao, Liu Nai-Cui, Chen Zhencheng, Han Guo-Cheng, Feng Xiao-Zhen, Kraatz Heinz-Bernhard
School of Life and Environmental Sciences, Guangxi Human Physiological, Information Non Invasive Detection Engineering Technology Research Center, Guangxi Key Laboratory of Metabolic Reprogramming and Intelligent Medical, Engineering for Chronic Diseases, Guangxi Colleges and Universities Key Laboratory of Biomedical Sensors and Intelligent Instruments, Guilin University of Electronic, Technology, Guilin, 541004, PR China.
School of Life and Environmental Sciences, Guangxi Human Physiological, Information Non Invasive Detection Engineering Technology Research Center, Guangxi Key Laboratory of Metabolic Reprogramming and Intelligent Medical, Engineering for Chronic Diseases, Guangxi Colleges and Universities Key Laboratory of Biomedical Sensors and Intelligent Instruments, Guilin University of Electronic, Technology, Guilin, 541004, PR China.
Talanta. 2025 Sep 4;297(Pt B):128797. doi: 10.1016/j.talanta.2025.128797.
An one-pot method was used to prepare bimetallic nanozymes, with chitosan (CS) and l-tyrosine (L-Tyr) as stabilized dispersed colloidal solutions and a carrier for gold-platinum single atoms (Au-Pt SAs), which exhibited excellent peroxidase activity. A colorimetric method based on CS/L-Tyr/Au-Pt SAs nanozymes was constructed for the colorimetric detection of quercetin (QR) in human serum and orange juice. The synthesized bimetallic nanozymes were characterized by SEM, TEM, HAADF-STEM, FT-IR, XRD and XPS techniques to demonstrate the successful synthesis of CS/L-Tyr/Au-Pt SAs nanozymes. Due to the extremely high catalytic activity of the intrinsic active sites of Au-Pt SAs, each site can effectively catalyze the decomposition of HO to produce hydroxyl radicals (·OH), which oxidizes the colorless substrate 3,3',5,5'-tetramethylbenzidine (TMB) to produce a blue product (ox-TMB), and the blue product gradually disappeared after the addition of QR, which indicates that QR can effectively inhibit the TMB oxidation of TMB. In addition, the effect of various experimental conditions on the activity of the nanozymes was investigated to obtain the optimal enzyme activity. The high selectivity of the nanozymes for QR detection was verified by using analogs as interfering agents. The linear range of this colorimetric method for the detection of QR was 0.001-500.0 μM with a detection limit of 0.33 nM. It was successfully used for QR detection in real samples of human serum and orange juice with recoveries of 97.95 %-99.52 % and 97.18 %-105.04 %, respectively, proving its great value for practical applications in biological systems.
采用一锅法制备双金属纳米酶，以壳聚糖（CS）和L-酪氨酸（L-Tyr）作为稳定的分散胶体溶液以及金-铂单原子（Au-Pt SAs）的载体，该双金属纳米酶表现出优异的过氧化物酶活性。构建了基于CS/L-Tyr/Au-Pt SAs纳米酶的比色法，用于比色检测人血清和橙汁中的槲皮素（QR）。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、高角度环形暗场扫描透射电子显微镜（HAADF-STEM）、傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）和X射线光电子能谱（XPS）技术对合成的双金属纳米酶进行表征，以证明CS/L-Tyr/Au-Pt SAs纳米酶的成功合成。由于Au-Pt SAs固有活性位点具有极高的催化活性，每个位点都能有效催化H₂O₂分解产生羟基自由基（·OH），羟基自由基将无色底物3,3',5,5'-四甲基联苯胺（TMB）氧化生成蓝色产物（ox-TMB），加入QR后蓝色产物逐渐消失，这表明QR能有效抑制TMB的氧化。此外，研究了各种实验条件对纳米酶活性的影响，以获得最佳酶活性。以类似物作为干扰剂验证了纳米酶对QR检测的高选择性。该比色法检测QR的线性范围为0.001 - 500.0 μM，检测限为0.33 nM。该方法成功用于人血清和橙汁实际样品中QR的检测，回收率分别为97.95% - 99.52%和97.18% - 105.04%，证明了其在生物系统实际应用中的巨大价值。
