Ishimitsu T, Hirose S
Anal Biochem. 1985 Nov 1;150(2):300-8. doi: 10.1016/0003-2697(85)90514-7.
In a study of in vitro and in vivo metabolism of L-3,4-dihydroxyphenylalanine (L-Dopa), two methods of high-performance liquid chromatography (HPLC) were used to separate the m- and p-O-methylated products. A reversed-phase column and an aqueous mobile phase by gradient elution were used; the elute was analyzed electrochemically with a single amperometric and dual coulometric electrode. The L-Dopa and its O-methylated products could be detected individually in the enzymatic methylation of rat liver homogenate and in patients with Parkinson's disease. Meta/para ratios of O-methylation are easily obtained by this method.
在一项关于L-3,4-二羟基苯丙氨酸（L-多巴）体外和体内代谢的研究中，使用了两种高效液相色谱（HPLC）方法来分离间位和对位O-甲基化产物。采用反相柱和梯度洗脱的水性流动相；洗脱液通过单安培和双库仑电极进行电化学分析。在大鼠肝脏匀浆的酶促甲基化过程以及帕金森病患者中，L-多巴及其O-甲基化产物能够被单独检测出来。通过这种方法可以轻松获得O-甲基化的间位/对位比率。
