Bartold P M, Page R C
Anal Biochem. 1985 Nov 1;150(2):320-4. doi: 10.1016/0003-2697(85)90517-2.
A simple and reliable method is described for the determination of glycosaminoglycans and proteoglycans in tissue extracts as well as during preparative and analytical procedures for these molecules. It is particularly useful because it requires much less starting material for the identification of glycosaminoglycans or proteoglycans and, in addition, is several fold more sensitive than the currently used uronic acid assays. The procedure allows separation of macromolecules by ion-exchange chromatography, density gradient centrifugation, or molecular sieve chromatography and involves spotting onto cellulose acetate membrane, reaction with Alcian blue, and quantitation of color in a spectrophotometer. This method is particularly appropriate to use for the analysis of proteoglycans and glycosaminoglycans in tissues which are available in limited amounts or have low levels of these macromolecules.
本文描述了一种简单可靠的方法，用于测定组织提取物中以及这些分子的制备和分析过程中的糖胺聚糖和蛋白聚糖。该方法特别有用，因为它鉴定糖胺聚糖或蛋白聚糖所需的起始材料要少得多，此外，其灵敏度比目前使用的糖醛酸测定法高几倍。该方法可以通过离子交换色谱、密度梯度离心或分子筛色谱分离大分子，包括点样到醋酸纤维素膜上、与阿尔辛蓝反应以及在分光光度计中对颜色进行定量。这种方法特别适用于分析数量有限或这些大分子含量低的组织中的蛋白聚糖和糖胺聚糖。
