一种将强碱性蛋白质从十二烷基硫酸钠-聚丙烯酰胺凝胶高效印迹至硝酸纤维素膜上的方法。
A method for the efficient blotting of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose.
作者信息
Szewczyk B, Kozloff L M
出版信息
Anal Biochem. 1985 Nov 1;150(2):403-7. doi: 10.1016/0003-2697(85)90528-7.
PMID:4091265
Abstract
An improved procedure for the electrophoretic transfer of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose is described. The use of more alkaline transfer buffers and the omission of an equilibration step before the transfer allow for the almost complete transfer of strongly basic proteins from gels to nitrocellulose without lowering the transfer efficiency for other proteins.
摘要
本文描述了一种改进的方法，用于将强碱性蛋白质从十二烷基硫酸钠-聚丙烯酰胺凝胶电泳转移至硝酸纤维素膜上。使用碱性更强的转移缓冲液并在转移前省略平衡步骤，可使强碱性蛋白质几乎完全从凝胶转移至硝酸纤维素膜，同时又不会降低其他蛋白质的转移效率。
Zotero 插件