通过亲和色谱法从氧化还原酶中分离细菌荧光素酶。
Separation of bacterial luciferase from oxidoreductases by affinity chromatography.
作者信息
Tsai T S
出版信息
Anal Biochem. 1985 Nov 15;151(1):137-41. doi: 10.1016/0003-2697(85)90063-6.
PMID:4091272
Abstract
The NADH-dependent and NADPH-dependent oxidoreductase activities associated with bacterial luciferase in Vibrio (Beneckea) harveyi can simultaneously be removed from purified luciferase through a Blue Sepharose CL-6B column. The result achieved with this one-step affinity chromatography is similar to that obtained with two sequential "reverse-affinity" chromatographies.
摘要
哈维弧菌（贝内克氏菌）中与细菌荧光素酶相关的依赖NADH和依赖NADPH的氧化还原酶活性，可通过蓝色琼脂糖凝胶CL - 6B柱同时从纯化的荧光素酶中去除。这种一步亲和层析所获得的结果与通过两步连续“反亲和”层析所获得的结果相似。
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