循环中的溶质载体家族17成员5（SLC17A5）作为年轻血脂异常个体早期内皮功能障碍的诊断生物标志物。
Circulating SLC17A5 as a Diagnostic Biomarker of Early Endothelial Dysfunction in Young Dyslipidemic Individuals.
作者信息
Akhtar Shamima, Sagar Komal, Hote Milind P, Roy Ambuj, Yadav Savita, Sharma Alpana
机构信息
Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), Room No. 3048, Ansari Nagar, New Delhi, 110029, India.
Department of Biophysics, AIIMS, New Delhi, India.
出版信息
J Cardiovasc Transl Res. 2025 Sep 5. doi: 10.1007/s12265-025-10689-w.
Diagnostic potential of sialin in identifying endothelial dysfunction is explored. 50 CAD patients, 50 young (20-35 years) dyslipidemic individuals (DLP), and 50 healthy controls (HC) were included in the study. HUVECs were stimulated with either TNFα or AT-2. RNA isolation, Real-time PCR, ELISA, and immunofluorescence staining were performed. In silico analysis was performed. ROC curves were constructed. Stimulated ECs showed increased sialin mRNA expression. Sialin mRNA peaked in the supernatant at 1-6 h, decreasing by 24 h. Serum sialin mRNA was significantly higher in DLP patients than in HC and CAD patients, whereas CXCL14 mRNA was elevated in CAD patients. Sialin mRNA had high sensitivity/specificity for predicting endothelial dysfunction. In silico analysis revealed the binding of translational repressor RNPs to the 5'UTR of sialin mRNA. This is the first study highlighting circulating sialin mRNA as a novel biomarker for endothelial activation.
探索了唾液酸转运蛋白在识别内皮功能障碍方面的诊断潜力。该研究纳入了50例冠心病患者、50例年轻（20 - 35岁）血脂异常个体（DLP）和50例健康对照（HC）。用TNFα或AT - 2刺激人脐静脉内皮细胞（HUVECs）。进行了RNA分离、实时定量聚合酶链反应（Real - time PCR）、酶联免疫吸附测定（ELISA）和免疫荧光染色。进行了计算机分析。构建了受试者工作特征（ROC）曲线。刺激后的内皮细胞显示唾液酸转运蛋白信使核糖核酸（mRNA）表达增加。唾液酸转运蛋白mRNA在1 - 6小时时在上清液中达到峰值，到24小时时下降。DLP患者血清唾液酸转运蛋白mRNA显著高于HC和冠心病患者，而CXCL14 mRNA在冠心病患者中升高。唾液酸转运蛋白mRNA对预测内皮功能障碍具有高敏感性/特异性。计算机分析揭示了翻译抑制核糖核蛋白（RNPs）与唾液酸转运蛋白mRNA的5'非翻译区（5'UTR）的结合。这是第一项强调循环唾液酸转运蛋白mRNA作为内皮激活新生物标志物的研究。
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