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[与触诊法相比,利用血清和乳清通过被动血凝试验和酶联免疫吸附测定法对牛皮蝇蛆病进行免疫诊断的优势]

[Advantages of the immunodiagnosis of bovine hypodermosis established by passive hemagglutination and by ELISA, using serum and lactoserum, over the warble count].

作者信息

Boulard C

出版信息

Ann Rech Vet. 1985;16(4):335-43.

PMID:4091487
Abstract

For an immunodiagnosis of hypodermosis, the most favourable period, December to April, was determined by means of annual kinetics of the anti-Hypoderma antibodies. From 69 blood samples, immunodiagnosis by means of a passive hemagglutination and ELISA are strictly correlated (r = 0.429, P less than 0.01). The results obtained by the ELISA technique on the serum and lactoserum of 23 cows are also correlated (r = 0.444, P less than 0.02). The immunodiagnosis of hypodermosis carried out on the serum or the lactoserum requires a single sample, contrary to the warble count which demands numerous interventions in order to be viable.

摘要

对于皮下蝇蛆病的免疫诊断,通过抗皮下蝇抗体的年度动力学确定了最适宜的时期,即12月至4月。在69份血样中,采用被动血凝试验和酶联免疫吸附测定法进行免疫诊断的结果高度相关(r = 0.429,P < 0.01)。对23头奶牛的血清和乳清进行酶联免疫吸附测定法所得结果也具有相关性(r = 0.444,P < 0.02)。与需要多次操作才能进行的牛蝇蛆计数相反,利用血清或乳清进行皮下蝇蛆病的免疫诊断仅需一份样本。

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