Milhaud G, Rivière F, Enriquez B
Ann Rech Vet. 1985;16(4):369-77.
Eighty-four lambs weaned at birth were randomised to three groups, each of 28 animals. The amount of fluoride contained in the feed was carefully assessed. Two groups were administered with sodium fluoride. The dose was calculated so as to administer a quantity of fluoride close to the quantity that lambs would ingest in consuming alfalfa containing from 125 to 250 ppm fluoride. The daily amount of fluoride administered to the 1st group rose progressively between week 3 and week 8 from 0.45 to 2 mg/kg/day, then remained fixed until the animals were slaughtered. Group 2 received double amounts: from 0.90 mg/kg/day to 4 mg/kg/day between week 3 and week 8, at which level the amount was fixed. The treatment had no effect on the growth of the lambs: at week 17 their average weight was about 29 kg. Plasma levels of ionic fluorine rose steadily up to week 9, at which they remained fixed at about 0.40 mg/l in group 1 and 0.75 mg/l in group 2, whereas they ranged between 0.10 and 0.15 mg/l in the controls. Ionic fluorine averaged 28% of the total fluoride in the controls, 42% in group 1 and 47% in group 2. Fluoride levels in the bones were very high (up to 2760 ppm in the mandibles in group 2). Reversely, fluoride levels in the muscles were very low: 1.00 ppm in group 2. The biochemical paremeters calcium, phosphorus, magnesium, transaminases, phosphatases and gamma glutamyl transferases greatly varied in relation to the age of the animal but were not affected by fluoride intake.
84只出生时断奶的羔羊被随机分为三组，每组28只。仔细评估了饲料中所含氟化物的量。两组给予氟化钠。计算剂量以便给予的氟化物量接近羔羊在食用含125至250 ppm氟化物的苜蓿时摄入的量。第1组每天给予的氟化物量在第3周和第8周之间从0.45毫克/千克/天逐渐增加到2毫克/千克/天，然后保持固定直至动物被屠宰。第2组给予双倍剂量：在第3周和第8周之间从0.90毫克/千克/天增加到4毫克/千克/天，该剂量在该水平保持固定。该处理对羔羊的生长没有影响：在第17周时它们的平均体重约为29千克。离子氟的血浆水平在第9周之前稳步上升，在第9周时，第1组保持固定在约0.40毫克/升，第2组保持固定在约0.75毫克/升，而对照组则在0.10至0.15毫克/升之间。离子氟在对照组中平均占总氟化物的28%，在第1组中占42%，在第2组中占47%。骨骼中的氟化物水平非常高（第2组下颌骨中高达2760 ppm）。相反，肌肉中的氟化物水平非常低：第2组为1.00 ppm。钙、磷、镁、转氨酶、磷酸酶和γ-谷氨酰转移酶等生化参数随动物年龄有很大变化，但不受氟化物摄入量的影响。
