Thevenet A
Arch Anat Microsc Morphol Exp. 1985;74(2):121-32.
A cell proliferation study during in vitro wound healing of dorsal thoraco-lumbar skin of 7-day chick embryos was performed by pulse labelling using a single isotope (tritiated thymidine). The unoperated (controls) and operated explants were incubated in the radioactive medium (1 microCi/ml tritiated thymidine) 1 h prior to fixation and where fixed 1 h (start control), 48, 72 and 96 h after the excision. Mean labelling and mitotic indices of the unwounded epidermis were, respectively, 18.22% and 2.66% at 7 days, and 7.03% and 0.88% at 11 days (7 days + 96 h). 72 h after the excision, the labelling and mitotic indices of wounded epidermis increased, on average, respectively to 212,5% and 220% with respect to those of the controls, in the proximal zones near the inner edge on the wound. The labelling and mitotic indices in the dermis were, respectively, 27.95% and 3.63% at 7 days and 7.65% and 1.30% at 11 days. 72 h after the excision, the labelling and mitotic indices of the operated dermis increased, on average, respectively to 220% and 130% with respect to those of the controls in the centre and the proximal zones of the wound. The increase of the labelling index of the operated integument persisted for a maximum of 24 h, between 48 to 72 hours after the excision.
利用单一同位素（氚标记胸腺嘧啶核苷）脉冲标记法，对7日龄鸡胚胸腰背部皮肤进行体外伤口愈合过程中的细胞增殖研究。在固定前1小时，将未手术（对照）和手术外植体置于放射性培养基（1微居里/毫升氚标记胸腺嘧啶核苷）中培养，并在切除后1小时（起始对照）、48、72和96小时进行固定。未受伤表皮的平均标记指数和有丝分裂指数在7天时分别为18.22%和2.66%，在11天（7天+96小时）时分别为7.03%和0.88%。切除后72小时，伤口表皮的标记指数和有丝分裂指数在伤口内边缘附近的近端区域平均分别相对于对照增加到212.5%和220%。真皮的标记指数和有丝分裂指数在7天时分别为27.95%和3.63%，在11天时分别为7.65%和1.30%。切除后72小时，手术真皮的标记指数和有丝分裂指数在伤口中心和近端区域平均分别相对于对照增加到220%和130%。手术皮肤标记指数的增加在切除后48至72小时之间最多持续24小时。
