犬在静息状态以及基于渗透压和非渗透压刺激试验后的血浆精氨酸加压素和血清 copeptin 浓度

Plasma Arginine Vasopressin and Serum Copeptin Concentrations at Rest and After Osmotic- and Non-Osmotic-Based Stimulation Tests in Dogs.

作者信息

Paulin Mathieu Victor, Schermerhorn Thomas, Mehrabanpour Dorsa, Unniappan Suraj, Snead Elisabeth

机构信息

Department of Small Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.

Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.

出版信息

J Vet Intern Med. 2025 Sep-Oct;39(5):e70219. doi: 10.1111/jvim.70219.

DOI:10.1111/jvim.70219

PMID:40916655

Abstract

BACKGROUND

Serum copeptin (sCoP) is used as a surrogate for plasma arginine vasopressin (pAVP) measurement in humans.

OBJECTIVE

To measure pAVP and sCoP at rest and after osmotic- and non-osmotic stimulation testing in dogs.

ANIMALS

Eight young castrated/spayed healthy research Beagles, eight young intact dogs, and eight old neutered healthy client-owned dogs.

METHODS

In this prospective longitudinal study, pAVP and sCoP were measured under iso-(baseline), hypo-(water load followed by intravenous administration of desmopressin [WLT]), and hyper-(water deprivation test [WDT]) osmolar conditions assessed by measured plasma osmolality (pOsm), and after administration of arginine (AST), IV, and Bovril (BST), PO. The fraction of change (F) in a variable y (e.g., pAVP) between baseline (T) and a timepoint X (T) during testing was defined as Fy = [y (T) - y (T)]/y (T).

RESULTS

Baseline sCoP had wide inter-individual variations. Mean [range] FpAVP and FsCoP at the end of WDT were +110% [+80; +142] and +18% [+0.4; +38] compared to baseline, respectively. Mean [range] FpAVP after water load and FsCoP after water load followed by desmopressin administration were -22% [-48; -0.5] and -29% [-39; -14] compared to baseline, respectively. Both FpAVP and FsCoP were strongly correlated to FpOsm (r = +0.76, p = 0.004; r = +0.78, p = 0.002; respectively). When sCoP was measured at T instead of T during WLT, to reflect its longer half-life reported in humans, the correlation between FpAVP and FsCoP became excellent (r = +0.90, p < 0.001). No stimulation of sCoP secretion occurred during AST or BST.

CONCLUSION AND CLINICAL IMPORTANCE

Serum CoP could be used as a surrogate for pAVP measurement in healthy dogs.

摘要

背景

血清 copeptin（sCoP）在人类中用作血浆精氨酸加压素（pAVP）测量的替代指标。

目的

在犬类静息状态以及渗透和非渗透刺激试验后测量 pAVP 和 sCoP。

动物

8 只年轻去势/绝育的健康实验用比格犬、8 只年轻未绝育的犬以及 8 只老年去势的健康客户拥有的犬。

方法

在这项前瞻性纵向研究中，在等渗（基线）、低渗（水负荷后静脉注射去氨加压素 [WLT]）和高渗（禁水试验 [WDT]）渗透压条件下，通过测量血浆渗透压（pOsm）来测量 pAVP 和 sCoP，并且在静脉注射精氨酸（AST）、静脉注射以及口服 Bovril（BST）后进行测量。测试期间，变量 y（例如 pAVP）在基线（T）和时间点 X（T）之间的变化分数（F）定义为 Fy = [y（T） - y（T）]/y（T）。

结果

基线 sCoP 存在较大的个体间差异。与基线相比，WDT 结束时 FpAVP 和 FsCoP 的平均值[范围]分别为 +110%[+80；+142]和 +18%[+0.4；+38]。水负荷后 FpAVP 的平均值[范围]以及水负荷后注射去氨加压素后 FsCoP 的平均值[范围]与基线相比分别为 -22%[-48；-0.5]和 -29%[-39；-14]。FpAVP 和 FsCoP 均与 FpOsm 高度相关（r = +0.76，p = 0.004；r = +0.78，p = 0.002）。在 WLT 期间，当在 T 而非 T 测量 sCoP 以反映其在人类中报道的较长半衰期时，FpAVP 和 FsCoP 之间的相关性变得极佳（r = +0.90，p < 0.001）。在 AST 或 BST 期间未发生 sCoP 分泌的刺激。