Miadelets O D
Arkh Anat Gistol Embriol. 1985 Dec;89(12):81-6.
The skin of non-inbred white rats has been studied from birth up to 2 years of age. The amount of non-pigmented granular dendrocytes (AGD) (Langerhans cells) has been determined in the epidermis by means of reaction to ATP-as and lymphocytes. Mitotic activity (MA), DNA contents in basal keratinocytes, enzymatic activity of the energy metabolism in the derma of the papillary layer, amount of tissue basophils and presence of serotonin in them are estimated by means of the luminescent method. During the first month of life the amount of the AGD and lymphocytes in epidermis increases and there is a parallel decrease in the MA, in thickness of epidermis, in DNA contents of the basal keratinocytes. The distribution of the enzymatic activity of the energy metabolism in the epidermis becomes similar to that in mature animals. By the old age the amount of the AGD and lymphocytes decreases. The amount of the tissue basophils increases up to the 19th day of life, then it decreases, and especially noticeably--by the old age. Serotonin is revealed for the first time in the tissue basophils at the age of 16 days. Its contents increases quickly during the first month of life and remains constant up to the end of the observations. There is a certain parallelism in amount of AGD, epidermal lymphocytes and tissue basophils of the derma. Perhaps in the skin there is a local functional system: basal keratinocytes--AGD--intraepidermal lymphocytes--tissue basophil of the derma.
对非近亲繁殖的白鼠从出生到2岁的皮肤进行了研究。通过对ATP酶和淋巴细胞的反应，测定了表皮中无色素颗粒状树突状细胞（AGD，即朗格汉斯细胞）的数量。采用发光法估计有丝分裂活性（MA）、基底角质形成细胞中的DNA含量、乳头层真皮中能量代谢的酶活性、组织嗜碱性粒细胞的数量以及其中血清素的存在情况。在出生后的第一个月，表皮中AGD和淋巴细胞的数量增加，同时MA、表皮厚度以及基底角质形成细胞的DNA含量则相应减少。表皮中能量代谢的酶活性分布变得与成熟动物相似。到老年时，AGD和淋巴细胞的数量减少。组织嗜碱性粒细胞的数量在出生后第19天前增加，之后减少，在老年时尤其明显减少。血清素在16日龄的组织嗜碱性粒细胞中首次被发现。其含量在出生后的第一个月迅速增加，并在观察结束前保持恒定。皮肤中的AGD、表皮淋巴细胞和真皮组织嗜碱性粒细胞的数量之间存在一定的平行关系。或许在皮肤中存在一个局部功能系统：基底角质形成细胞——AGD——表皮内淋巴细胞——真皮组织嗜碱性粒细胞。
