Pentilla I A, Ey P L, Lopez A F, Jenkin C R
Aust J Exp Biol Med Sci. 1985 Oct;63 ( Pt 5):531-43. doi: 10.1038/icb.1985.57.
The role of neutrophils and eosinophils in the acquired resistance of mice to infection with the helminth Nematospiroides dubius has been investigated in vivo by testing infected mice for their resistance to a challenge dose of larvae following treatment with monoclonal rat antibodies (MAbs) specific for mouse granulocytes. Treatment of normal and infected mice with NIMP-R10 MAb reduced the number of neutrophils in the peripheral blood and peritoneal cavity during the ensuing 2-5 days without affecting macrophage or eosinophil levels. The effect of this MAb on immunity depended on the immune status of the mice tested. The partial resistance of mice receiving a primary 'immunising' infection, followed by passive transfer of immune serum and challenge at 4 days, was completely suppressed by NIMP-R10 MAb. The acquired resistance of mice challenged 10 days after being given a single 'immunising' infection was halved by NIMP-R10 treatment, while that of 'twice-immunised' mice (infected on days 0 and 14, challenged on day 24) was unaffected. Treatment of twice-infected mice with the eosinophil-specific MAb NIMP-R6 reduced the number of eosinophils in the blood and peritoneal cavity by 40-60%, but caused no significant loss of immunity. The data indicate that after infection of mice with N. dubius, neutrophils play a predominant role in early resistance to reinfection, but they become progressively less essential as activated macrophages and (following a second infection) eosinophils become prevalent. The lack of effect of NIMP-R6 MAb treatment on the immunity of twice-infected mice may have been due to an insufficient reduction in eosinophil numbers; however, it seems likely that the neutrophils and macrophages present in these mice would have provided adequate protection even in the absence of eosinophils.
通过用针对小鼠粒细胞的单克隆大鼠抗体（MAb）处理感染的小鼠，然后检测它们对幼虫攻击剂量的抵抗力，在体内研究了中性粒细胞和嗜酸性粒细胞在小鼠对蠕虫双睾旋尾线虫感染的获得性抵抗力中的作用。用NIMP-R10 MAb处理正常和感染的小鼠，在随后的2-5天内降低了外周血和腹腔中的中性粒细胞数量，而不影响巨噬细胞或嗜酸性粒细胞水平。这种MAb对免疫的影响取决于所测试小鼠的免疫状态。接受初次“免疫”感染、随后被动转移免疫血清并在4天时进行攻击的小鼠的部分抵抗力被NIMP-R10 MAb完全抑制。在单次“免疫”感染后10天受到攻击的小鼠的获得性抵抗力通过NIMP-R10处理减半，而“两次免疫”小鼠（在第0天和第14天感染，在第24天攻击）的抵抗力不受影响。用嗜酸性粒细胞特异性MAb NIMP-R6处理两次感染的小鼠，使血液和腹腔中的嗜酸性粒细胞数量减少40-60%，但未导致免疫力显著丧失。数据表明，小鼠感染双睾旋尾线虫后，中性粒细胞在早期抵抗再感染中起主要作用，但随着活化的巨噬细胞和（第二次感染后）嗜酸性粒细胞变得普遍，它们的重要性逐渐降低。NIMP-R6 MAb处理对两次感染小鼠免疫力缺乏影响可能是由于嗜酸性粒细胞数量减少不足；然而，即使没有嗜酸性粒细胞，这些小鼠中存在的中性粒细胞和巨噬细胞似乎也能提供足够的保护。
