Luo Guangping, Zhang Tongtong, Jiang Yanan, Qin Yuan, Chen Pengfei, Hu Junqing
Obesity and Metabolism Medicine-Engineering Integration Laboratory, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu, China.
The Center for Obesity and Metabolic Diseases, Department of General Surgery, The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu, China.
Front Bioeng Biotechnol. 2025 Aug 21;13:1584976. doi: 10.3389/fbioe.2025.1584976. eCollection 2025.
Salecan-based hydrogel (thereafter called Sal-hydrogel) dressings and gut microbiota have been associated with enhanced wound healing. However, the relationship between these two factors remains unclear. This study investigated the dynamic characteristics of the intestinal microbiota in relation to the Sal-hydrogel dressings and their role in promoting wound healing. Fecal samples were collected at day 0, 3, 7, and 12 after wounds were inflicted on 48 mice, which were treated with either Sal-hydrogel, , or a combination of and Sal-hydrogel, using a full-thickness skin perforation wound model. The samples were subjected to 16S rRNA V3-V4 gene sequencing. The results indicated a general trend for Shannon diversity of the intestinal microbiota to increase by day 3 following injury. On the final day, the diversity in both the hydrogel group and the plus hydrogel group was significantly higher compared to the control group ( < 0.05). Additionally, the bacterial community structure in the mouse gut exhibited substantial changes when comparing the hydrogel-treated groups to the controls (hydrogel group: = 0.027; plus hydrogel group: = 0.039). The genus Oscillospiraceae, which was significantly associated with wound closure (R-squared = 0.2154, = 6.657e-05), was found to be dominant in the gut of the hydrogel group during the wound healing process. Overall, our findings suggest that significant and rapid alterations in gut microbiota occur in response to skin injury and wound infection. The enhanced wound healing properties of the Sal-hydrogel dressing are associated with increased intestinal microbiota diversity and the presence of the bacterium Oscillospiraceae.
基于硫酸软骨素的水凝胶（以下简称Sal-水凝胶）敷料与肠道微生物群已被证实与促进伤口愈合有关。然而，这两个因素之间的关系仍不明确。本研究调查了与Sal-水凝胶敷料相关的肠道微生物群的动态特征及其在促进伤口愈合中的作用。使用全层皮肤穿孔伤口模型，对48只小鼠造成伤口后，在第0、3、7和12天收集粪便样本，这些小鼠分别用Sal-水凝胶、或与Sal-水凝胶联合处理。样本进行16S rRNA V3-V4基因测序。结果表明，受伤后第3天肠道微生物群的香农多样性总体呈增加趋势。在最后一天，水凝胶组和加了的水凝胶组的多样性均显著高于对照组（<0.05）。此外，与对照组相比，水凝胶处理组小鼠肠道中的细菌群落结构发生了显著变化（水凝胶组：=0.027；加了的水凝胶组：=0.039）。在伤口愈合过程中，与伤口闭合显著相关的颤螺菌科（R平方=0.2154，=6.657e-05）在水凝胶组的肠道中占主导地位。总体而言，我们的研究结果表明，肠道微生物群会因皮肤损伤和伤口感染而发生显著且快速的变化。Sal-水凝胶敷料增强的伤口愈合特性与肠道微生物群多样性增加以及颤螺菌科细菌的存在有关。 （注：原文中“,”处内容缺失，翻译时保留原样）
