Alrosan Mohammad, Al-Massad Motasem, Obeidat Hadeel J, Maghaydah Sofyan, Alu'datt Muhammad H, Tan Thuan-Chew, Liao Zhengrui, Alboqai Omar, Ebqa'ai Mohammad, Dheyab Mohammed Ali, Almajwal Ali Madi
Department of Food Science and Nutrition, Faculty of Agriculture, Jerash University, P.O. Box 311, Jerash, 26150 Jordan.
QU Health, College of Health Sciences, Qatar University, P.O. Box 2713, Doha, Qatar.
Food Sci Biotechnol. 2025 Jul 5;34(14):3317-3329. doi: 10.1007/s10068-025-01930-y. eCollection 2025 Oct.
This research study investigates the relationship between the structural characteristics, water solubility, and protein digestibility of quinoa proteins (QPs) during fermentation. The fermentation process induces structural modifications in QPs, thereby enhancing their surface properties and functional attributes. Using advanced analytical techniques, such as ultraviolet, fluorescence, and FT-IR spectra, it has been demonstrated that fermented QPs exhibit significant structural changes (P < 0.05) compared to unfermented QPs. Notably, fermentation significantly increases the digestibility of QPs from 78.13 to 85.24%, thereby enhancing their nutritional value. Furthermore, surface properties undergo modifications, with a decrease in surface charge from -32.82 to -39.63 and a reduction in surface hydrophobicity from 580 to 382 a.u. These findings underscore the transformative impact of fermentation on QPs, resulting in improved digestibility, modifications in protein structure, and enhanced nutritional benefits. Further research on water kefir-based fermentation is needed to optimize its quality.
本研究调查了藜麦蛋白(QPs)在发酵过程中的结构特征、水溶性和蛋白质消化率之间的关系。发酵过程会引起QPs的结构修饰,从而增强其表面性质和功能特性。使用先进的分析技术,如紫外、荧光和傅里叶变换红外光谱,已证明与未发酵的QPs相比,发酵后的QPs表现出显著的结构变化(P < 0.05)。值得注意的是,发酵显著提高了QPs的消化率,从78.13%提高到85.24%,从而提高了它们的营养价值。此外,表面性质也发生了改变,表面电荷从-32.82降低到-39.63,表面疏水性从580 a.u.降低到382 a.u.。这些发现强调了发酵对QPs的变革性影响,导致消化率提高、蛋白质结构改变和营养益处增强。需要对基于水开菲尔的发酵进行进一步研究以优化其品质。