Nong Jiahuan, Li Pengcheng, Zhu Mingming, Li Yifeng, Wang Changsheng, Zhang Yutao, Yan Chenxu, Guo Zhiqian
Shanghai Key Laboratory of Functional Materials Chemistry, Institute of Fine Chemicals, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology Shanghai 200237 China
Division of Gastroenterology and Hepatology, NHC Key Laboratory of Digestive Diseases, Shanghai Institute of Digestive Disease, Inflammatory Bowel Disease Research Center, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai China
Chem Sci. 2025 Aug 28. doi: 10.1039/d5sc05331e.
Real-time monitoring of senescent cells is of great significance for understanding and intervening in aging. Since overexpression of endogenous β-galactosidase (β-gal) is not unique to senescent cells, probes relying solely on β-gal activity could yield inaccurate senescent cell detection. Herein, we designed a dual-mode sequential response AND logic NIR probe MFB-βgal, which contains a β-gal-cleavable unit and a morpholine unit, serving as an enzymatic activity trigger and a lysosomal targeting moiety, respectively. MFB-OH is generated after reaction with β-gal, which can detect the alkalinization of lysosomes by emission intensity in senescent cells. This probe has been successfully used to distinguish between SKOV-3 and senescent cells and applied to visualization of β-gal activity in a mouse model, providing a new strategy for the accurate detection of cellular senescence.
对衰老细胞进行实时监测对于理解和干预衰老具有重要意义。由于内源性β-半乳糖苷酶（β-gal）的过表达并非衰老细胞所特有，仅依赖β-gal活性的探针可能会导致衰老细胞检测不准确。在此，我们设计了一种双模式顺序响应与逻辑近红外探针MFB-βgal，它包含一个β-gal可裂解单元和一个吗啉单元，分别作为酶活性触发剂和溶酶体靶向部分。与β-gal反应后生成MFB-OH，它可以通过衰老细胞中的发射强度检测溶酶体的碱化。该探针已成功用于区分SKOV-3细胞和衰老细胞，并应用于小鼠模型中β-gal活性的可视化，为细胞衰老的准确检测提供了一种新策略。
