Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, China.
Department of Gastroenterology, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510145, China.
Anal Chem. 2023 Jul 18;95(28):10481-10485. doi: 10.1021/acs.analchem.3c01656. Epub 2023 Jul 3.
β-Galactosidase (β-gal) is the gold standard marker of cellular senescence, which is linked with various age-related diseases. Therefore, it is essential to develop more excellent probes that can real-time monitor β-gal activity in cellular senescence . Fluorescent/photoacoustic (FL/PA) dual-modal imaging possesses excellent sensitivity and spatial resolution. To our knowledge, there has been no tumor-targeted FL/PA probe to image cellular senescence by monitoring the activity of β-gal . Therefore, we developed a tumor-targeted FL/PA probe () for β-gal-activatable imaging of tumor senescence. without tumor-targeted biotin is used as a control probe. is superior to due to the higher kinetic parameter of than . Moreover, biotin could help enter and accumulate in tumor cells with higher FL/PA signal. In detail, or could image senescent tumor cells with 4.6-fold or 3.5-fold FL enhancement and 4.1-fold or 3.3-fold PA enhancement. or could image tumor senescence with 2.9-fold or 1.7-fold FL enhancement and 3.8-fold or 1.3-fold PA enhancement. We envision that will be applied for FL/PA imaging of tumor senescence in clinic.
β-半乳糖苷酶（β-gal）是细胞衰老的金标准标志物，与各种与年龄相关的疾病有关。因此，开发能够实时监测细胞衰老中β-gal 活性的更优秀的探针至关重要。荧光/光声（FL/PA）双模成像是一种具有优异灵敏度和空间分辨率的方法。据我们所知，还没有用于通过监测β-gal 活性来对细胞衰老进行成像的肿瘤靶向 FL/PA 探针。因此，我们开发了一种肿瘤靶向的 FL/PA 探针（），用于通过监测β-gal 的活性对肿瘤衰老进行荧光/光声双模式成像。没有肿瘤靶向生物素的作为对照探针。由于 比 的动力学参数更高，因此 优于 。此外，生物素可以帮助 进入并在具有更高 FL/PA 信号的肿瘤细胞中积累。具体来说，或 可以对衰老的肿瘤细胞进行成像，荧光增强 4.6 倍或 3.5 倍，光声增强 4.1 倍或 3.3 倍。或 可以对肿瘤衰老进行成像，荧光增强 2.9 倍或 1.7 倍，光声增强 3.8 倍或 1.3 倍。我们设想将 应用于临床肿瘤衰老的 FL/PA 成像。
