Xia Hailong, Wang Xianghe, Huang Weichun, Jiang Xindong, Han Xionggao, Wang Chaoyue
Orthopedics Department, Dongyang People's Hospital, Jinhua, China.
The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Front Chem. 2025 Aug 21;13:1666238. doi: 10.3389/fchem.2025.1666238. eCollection 2025.
In this work, a fluorescent probe, VanPI-CarE, with a vanillin-pyridine-imidazole core structure was developed for carboxylesterase (CarE) detection in macrophage polarization during bone homeostasis. The probe responded to CarE with a distinct fluorescence reporting signal at 490 nm upon excitation at 355 nm. Tests in solution showed the advantages of VanPI-CarE, including high sensitivity, excellent stability under various working conditions, high selectivity, and low cytotoxicity. Further confocal imaging indicated that VanPI-CarE enabled visualization of CarE level in living macrophages. The probe further revealed macrophage polarization in bone homeostasis under both induction and inhibition conditions via CarE detection. These findings provide meaningful insights for inflammation-related research.
在这项工作中，开发了一种具有香草醛 - 吡啶 - 咪唑核心结构的荧光探针VanPI - CarE，用于检测骨稳态过程中巨噬细胞极化时的羧酸酯酶（CarE）。该探针在355nm激发下，在490nm处产生明显的荧光报告信号以响应CarE。溶液测试显示了VanPI - CarE的优点，包括高灵敏度、在各种工作条件下的出色稳定性、高选择性和低细胞毒性。进一步的共聚焦成像表明，VanPI - CarE能够可视化活巨噬细胞中的CarE水平。该探针通过检测CarE进一步揭示了在诱导和抑制条件下骨稳态中的巨噬细胞极化。这些发现为炎症相关研究提供了有意义的见解。
