de Freitas Mileny Caroline Menezes, Volpato Leonardo Alex, Weber Vinicius Müller Reis, da Costa Julio Cesar, Martins Clarice Maria Lucena, Romanzini Marcelo, Mota Jorge, Ronque Enio Ricardo Vaz
Laboratory Physical Activity and Health, Center of Physical Education and Sport, State University of Londrina, Rodovia Celso Garcia Cid, PR-445, Km 380 - Campus Universitário, Londrina, Paraná, 86057-970, Brazil.
Laboratory of Experimental and Applied Physiology to Physical Activity, Midwest State University of Paraná, Guarapuava, Paraná, Brazil.
Eur J Pediatr. 2025 Sep 8;184(10):606. doi: 10.1007/s00431-025-06455-4.
The objective of this study is to analyze adherence to 24-h movement behavior recommendations (combined and isolated) with brain-derived neurotrophic factor (BDNF) in adolescents. For this cross-sectional study, 155 adolescents were recruited, of whom 141 participated; 118 with valid data were analyzed (64 girls, mean age 14.9 years). Sleep duration and screen time (ST) were self-reported, while moderate-to-vigorous physical activity (MVPA) was measured using accelerometers. These behaviors were classified based on adherence to the 24-h recommendations for adolescents. BDNF plasma levels were assessed using an enzyme-linked immunosorbent assay (ELISA) kit. Linear regression analyses were conducted to examine the associations between combinations of behaviors (MVPA + sleep, MVPA + ST, and sleep + ST) and individual behaviors and number of guidelines met (all vs one; all vs two; two vs one) in both crude and adjusted analyses. In the adjusted analyses, number of guidelines met all vs one (β = 75.42; [95% CI = 8.77; 142.08]) and two vs one (β = 76.73; [95% CI = 24.68; 128.78]) were positively associated with BDNF in boys. In girls, two vs one was associated with BDNF (β = 57.67; [95% CI = 10.62; 104.72). Furthermore, sleep adherence was associated with BDNF levels in girls (β = 72.33; [95% CI = 30.46; 114.20]) and boys (β = 46.73; [95% CI = 0.77; 92.69]). The combination of sleep + ST was also associated with BDNF levels in girls (β = 65.92; [95% CI = 23.83; 108.01]).
Adherence to at least two movement behaviors was associated with higher BDNF concentrations. Compliance with sleep recommendations showed a positive association with BDNF levels in both sexes. Among girls, the combination of sleep and ST within recommended levels was also associated with higher levels of the neurotrophic biomarker.
• The brain-derived neurotrophic factor (BDNF) signals and modulates synaptic interactions, influencing learning and memory. However, it also plays a critical role in the pathophysiology of depression. • Greater adherence to the 24-h movement guidelines is associated with favorable cognitive outcomes in adolescents. Nevertheless, studies examining BDNF in health adolescents remain limited.
• This study examined an important indicator of brain health, BDNF, and adherence to the 24-h movement guidelines in healthy adolescents. • The findings emphasize the role of sleep and screen time in being associated with higher circulating BDNF levels.
本研究的目的是分析青少年对24小时运动行为建议（综合及单独建议）的依从性与脑源性神经营养因子（BDNF）之间的关系。在这项横断面研究中，招募了155名青少年，其中141人参与；对118名有有效数据的青少年进行了分析（64名女孩，平均年龄14.9岁）。睡眠时间和屏幕使用时间（ST）通过自我报告获取，而中度至剧烈身体活动（MVPA）则使用加速度计进行测量。这些行为根据对青少年24小时建议的依从性进行分类。使用酶联免疫吸附测定（ELISA）试剂盒评估血浆BDNF水平。进行线性回归分析，以检验行为组合（MVPA+睡眠、MVPA+ST和睡眠+ST）与个体行为以及在粗分析和校正分析中达到的指南数量（全部与一项；全部与两项；两项与一项）之间的关联。在校正分析中，男孩达到的指南数量全部与一项（β=75.42；[95%CI=8.77；142.08]）以及两项与一项（β=76.73；[95%CI=24.68；128.78]）与BDNF呈正相关。在女孩中，两项与一项与BDNF相关（β=57.67；[95%CI=10.62；104.72]）。此外，睡眠依从性与女孩（β=72.33；[95%CI=30.46；114.20]）和男孩（β=46.73；[95%CI=0.77；92.69]）的BDNF水平相关。睡眠+ST的组合也与女孩的BDNF水平相关（β=65.92；[95%CI=23.83；108.01]）。
坚持至少两种运动行为与较高的BDNF浓度相关。遵守睡眠建议在两性中均与BDNF水平呈正相关。在女孩中，推荐水平内的睡眠和ST组合也与神经营养生物标志物的较高水平相关。
•脑源性神经营养因子（BDNF）发出信号并调节突触相互作用，影响学习和记忆。然而，它在抑郁症的病理生理学中也起着关键作用。•更好地遵守24小时运动指南与青少年良好的认知结果相关。然而，在健康青少年中研究BDNF的研究仍然有限。
•本研究调查了健康青少年中脑健康的一个重要指标BDNF以及对24小时运动指南的依从性。•研究结果强调了睡眠和屏幕使用时间与较高循环BDNF水平相关的作用。
