Di Gianvito Paola, Englezos Vasileios, Ferrocino Ilario, Cocolin Luca, Rantsiou Kalliopi
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo Braccini 2, 10095 Grugliasco, Italy.
Department of Agricultural, Forest and Food Sciences, University of Turin, Largo Braccini 2, 10095 Grugliasco, Italy; Interdepartmental Centre for Grapevines and Wine Sciences, University of Turin, Corso Enotria 2/C, 12051 Alba, Italy.
Food Res Int. 2025 Nov;219:117000. doi: 10.1016/j.foodres.2025.117000. Epub 2025 Jul 12.
Microorganisms colonizing grapevines possess diverse functional capabilities that influence the health, growth, productivity and, consequently, wine quality. In this study, spatial and temporal dynamics of the microbiome of Vitis vinifera cv. Barbera grapevine were determined by shotgun sequencing. Bacterial and fungal populations and functions were monitored in samples of rhizosphere, leaves, and grapes, collected at different stages from fruit development to harvest in a conventionally managed vineyard. A compartmental specificity of diverse species was observed within both bacterial and fungal communities. A core microbiome was also identified. LEfSe analysis revealed significantly discriminant taxa associated with each plant compartment, but not according to the sampling time. KEGG genes associated with carbohydrate metabolism were the most abundant in all samples, followed by genes related to amino acid metabolism, respectively involved in carbon and nitrogen metabolic pathways. Interestingly, differences were observed in the functions of rhizosphere and phyllosphere communities with additional differences observed between functions of bacterial and fungal communities. Pathways involved in critical functions like nutrient acquisition, stress resistance, metabolic flexibility, and interaction with the grapevine, were detected within the microbiome. The findings of this study unravel ecological and functional characteristics of the Barbera microbiome. This fundamental understanding is a prerequisite for the development of tailored strategies to protect vineyards and promote sustainability in grapevine production.
定殖于葡萄藤的微生物具有多种功能，这些功能会影响葡萄藤的健康、生长、生产力，进而影响葡萄酒的品质。在本研究中，通过鸟枪法测序确定了酿酒葡萄品种巴贝拉葡萄藤微生物组的时空动态。在一个传统管理的葡萄园里，从果实发育到收获的不同阶段，对根际、叶片和葡萄样本中的细菌和真菌种群及功能进行了监测。在细菌和真菌群落中均观察到不同物种的区室特异性。还鉴定出了一个核心微生物组。线性判别分析效应大小（LEfSe）分析揭示了与每个植物区室相关的显著判别分类群，但与采样时间无关。与碳水化合物代谢相关的京都基因和基因组百科全书（KEGG）基因在所有样本中最为丰富，其次是与氨基酸代谢相关的基因，它们分别参与碳和氮代谢途径。有趣的是，观察到根际和叶际群落功能存在差异，细菌和真菌群落功能之间也存在其他差异。在微生物组中检测到了参与养分获取、抗逆性、代谢灵活性以及与葡萄藤相互作用等关键功能的途径。本研究结果揭示了巴贝拉微生物组的生态和功能特征。这一基本认识是制定针对性策略以保护葡萄园并促进葡萄生产可持续性的先决条件。
