Yang Shiping, Feng Leyuan, Luo Junliang, Jian Jichang, Cai Shuanghu, Liu Huiling
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture and Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang, China.
Aquac Nutr. 2025 Aug 31;2025:6665220. doi: 10.1155/anu/6665220. eCollection 2025.
Nuclear factor erythroid 2-related factor 2 (Nrf2) is an essential component in regulating oxidative stress. Sulforaphane (SFN) is a natural antioxidant and gene agonist that can increase the antioxidant capacity of the organism and reduce oxidative stress. However, research on the repair of oxidative stress damage by SFN in aquatic animals remains extremely scarce. In order to further explore the function and role of SFN in the repair of oxidative stress injury in aquatic animals, this study took as the research object. We established an oxidative stress model of through 6% oxidized fish oil (OFO) feeding. Methods, such as RNA interference (RNAi), qPCR, histopathological analysis, and TUNEL assay, were used to detect the changes in the oxidative stress status of . The results showed that the expression of in the hepatopancreas of in the double-stranded RNA (dsRNA)- + SFN group was significantly higher than that in the dsRNA- group and control group at 24 h ( < 0.05). The transcription levels of antioxidant and autophagy genes in the SFN group were significantly higher than those in the control group ( < 0.05), and the expression of related genes in the dsRNA- + SFN group was also higher than that in the dsRNA- group. Histopathology showed that knockdown would aggravate hepatopancreatic apoptosis and vacuolation, while SFN treatment after knockdown could alleviate hepatopancreatic injury and apoptosis caused by OFO. The results indicated that SFN could repair the oxidative stress injury of induced by OFO by activating . This study investigated the role of SFN in alleviating and repairing the oxidative stress damage in caused by OFO, aiming to provide a theoretical basis for the research on the antioxidant effect of SFN and the regulation of the antioxidant capacity of shrimp.
核因子红细胞2相关因子2（Nrf2）是调节氧化应激的重要组成部分。萝卜硫素（SFN）是一种天然抗氧化剂和基因激动剂，可提高机体抗氧化能力并降低氧化应激。然而，关于SFN对水生动物氧化应激损伤修复作用的研究极为匮乏。为进一步探究SFN在水生动物氧化应激损伤修复中的功能和作用，本研究以[具体对象未给出]为研究对象。我们通过投喂6%氧化鱼油（OFO）建立了[具体对象未给出]的氧化应激模型。采用RNA干扰（RNAi）、qPCR、组织病理学分析和TUNEL检测等方法，检测[具体对象未给出]氧化应激状态的变化。结果显示，双链RNA（dsRNA）+SFN组[具体对象未给出]肝胰腺中[具体基因未给出]在24小时时的表达显著高于dsRNA组和对照组（P<0.05）。SFN组抗氧化和自噬基因的转录水平显著高于对照组（P<0.05），dsRNA+SFN组相关基因的表达也高于dsRNA组。组织病理学显示，[具体基因未给出]敲低会加重肝胰腺细胞凋亡和空泡化，而敲低后进行SFN处理可减轻OFO引起的肝胰腺损伤和细胞凋亡。结果表明SFN可通过激活[具体对象未给出]来修复OFO诱导的[具体对象未给出]氧化应激损伤。本研究探讨了SFN在减轻和修复OFO引起的[具体对象未给出]氧化应激损伤中的作用，旨在为SFN抗氧化作用及虾抗氧化能力调控的研究提供理论依据。
