通过血红蛋白珠蛋白链的等电聚焦法测定血红蛋白的非酶糖基化作用。

Determination of the non-enzymatic glycation of hemoglobin by isoelectrofocusing of its globin chains.

作者信息

Castagnola M, Caradonna P, Bertollini A, Cassiano L, Rossetti D V, Salvi M L

出版信息

Clin Biochem. 1985 Dec;18(6):327-31. doi: 10.1016/s0009-9120(85)80069-2.

Abstract

Micro cation exchange chromatography determination of HbA1c does not provide a complete picture of Hb glycation, for it does not determine all the glycated forms of hemoglobin. For the determination of total glycation, we describe here a rod IEF method, which allows the simultaneous quantitation of glycation on alpha and beta globin chains. The method exhibits good sensitivity; it is not affected by artifacts deriving from temperature, hypertriglyceridemia, Hb variants or labile HbA1 (aldiminic Hb). The results obtained indicate that in a normal population approximately 18% of the beta chain and 8% of the alpha chain are glycated. These mean percentages increase in the diabetic to 28% and 12%, respectively. The beta chain is glycated on both valine and lysine residues, while the alpha chain is glycated only on the latter. HbA1 values from micro cation exchange chromatography are significantly related to both alpha and beta glycation. Thus, valinic or lysinic glycation have roughly the same clinical significance.

摘要

微量阳离子交换色谱法测定糖化血红蛋白（HbA1c）并不能全面反映血红蛋白的糖基化情况，因为它无法测定所有糖化形式的血红蛋白。为了测定总糖基化情况，我们在此描述一种棒状等电聚焦（IEF）方法，该方法能够同时定量α和β珠蛋白链上的糖基化。该方法具有良好的灵敏度；不受温度、高甘油三酯血症、血红蛋白变异体或不稳定HbA1（醛亚胺型Hb）产生的假象影响。所得结果表明，在正常人群中，约18%的β链和8%的α链发生了糖基化。在糖尿病患者中，这些平均百分比分别增加到28%和12%。β链在缬氨酸和赖氨酸残基上均发生糖基化，而α链仅在赖氨酸残基上发生糖基化。微量阳离子交换色谱法测得的HbA1值与α和β糖基化均显著相关。因此，缬氨酸或赖氨酸糖基化具有大致相同的临床意义。