Zhao Mei, Chen Jiaojiao, Pan Xiaohong, Zabed Hossain M, Arsalan Abdullah, Qi Xianghui
School of Food & Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013 Jiangsu Province, China.
School of Life Sciences, Guangzhou University, 230 Wai Huan Xi Road, Guangzhou 510006 Guangdong, China.
J Agric Food Chem. 2025 Sep 17;73(37):23078-23097. doi: 10.1021/acs.jafc.5c06547. Epub 2025 Sep 9.
Pectinases are indispensable biocatalysts for pectin degradation in food and bioprocessing industries, yet natural enzymes often lack tailored functionalities for modern applications. While a previous review discussed pectinases in terms of production and application, this review particularly discusses an integrated framework for robust pectinases. This framework combines enzyme mining, protein engineering, and AI-assisted design to systematically discover, optimize, and customize pectinases. These synergistic strategies, in fact, have been widely explored in recent years to enable precise development of biocatalysts with enhanced industrial traits, moving beyond traditional single-approach-based enzyme improvement. Specifically, we discuss how cutting-edge methodologies, such as data-driven discovery and intelligent protein engineering, accelerate robust pectinase development, while emerging purification and bioprocessing techniques expand their applications in juice/wine production, textile bioscouring, and agricultural waste valorization. By unifying novel microbial sources, mechanistic insights, and engineering advances, these holistic approaches offer transformative potential for biocatalyst development, including pectinases. In this way, this review consolidates recent progress to guide next-generation pectinase development through combinatorial biotechnology, providing actionable insights for advancing sustainable industrial processes.
果胶酶是食品和生物加工行业中果胶降解不可或缺的生物催化剂，但天然酶往往缺乏适用于现代应用的定制功能。虽然之前的一篇综述从生产和应用方面讨论了果胶酶，但本综述特别讨论了用于高效果胶酶的综合框架。该框架结合了酶挖掘、蛋白质工程和人工智能辅助设计，以系统地发现、优化和定制果胶酶。事实上，近年来人们广泛探索了这些协同策略，以实现具有增强工业特性的生物催化剂的精确开发，超越了传统的基于单一方法的酶改进。具体而言，我们讨论了数据驱动发现和智能蛋白质工程等前沿方法如何加速高效果胶酶的开发，而新兴的纯化和生物加工技术则扩大了它们在果汁/葡萄酒生产、纺织品生物精练和农业废弃物增值利用方面的应用。通过整合新型微生物来源、机理见解和工程进展，这些整体方法为包括果胶酶在内的生物催化剂开发提供了变革潜力。通过这种方式，本综述巩固了近期的进展，以指导通过组合生物技术进行下一代果胶酶的开发，为推进可持续工业过程提供可操作的见解。
