Nowruzi Bahareh, Sadeghi AmirHossein, Meftahi Mohammad
Department of Biology, SR.C., Islamic Azad University, Tehran, Iran.
Odontology. 2025 Sep 9. doi: 10.1007/s10266-025-01188-0.
Streptococcus mutans, a key cause of dental caries, is not treated by conventional toothpaste, brushing, flossing, or antiseptic mouthwashes. This necessitates the development of enriched toothpaste. Cyanobacteria-derived phycoerythrin (PE) has antioxidant and antibacterial properties. Magnetic field (MF) treatments are also effective in cyanobacterial metabolism and antioxidant defense. This study aimed to develop toothpaste with Aliinostoc sp. pigment under MFs of 30 and 60 millitesla (mT), along with Oliveria decumbens Vent. oil, to evaluate its antioxidant and antibacterial properties. The findings of several assessments over 45 days on 5 varieties of toothpaste indicated that the 30 mT MF had the highest wavelength and PE absorption. In addition, the purity of PE in cultures treated with MFs rose considerably compared to the control. The findings of the average diameter of the growth inhibition zone of toothpaste treatments against S. mutans bacteria indicated that the combination of Oliveria decumbens Vent. oil and PE led to a substantial increase in antibacterial activity compared to the control treatment (1.5 times) (p < 0.05). The addition of PE and Oliveria decumbens Vent. oil under MFs reduced water loss (1.5 times), enhanced texture uniformity (2 times), increased phenolic content (2 times), boosted antioxidant activity, and lowered pH in toothpaste treatments compared to the control (p < 0.05). In addition, the findings of the sensory evaluation of the overall acceptability of various toothpaste treatments revealed that the combination of Oliveria decumbens Vent. oil and PE boosted the overall acceptability of toothpaste treatments compared to the control treatment (p<0.05). In general, the employment of PE under MFs and Oliveria decumbens Vent. oil in toothpaste formulations was particularly beneficial for reducing tooth decay and periodontal illnesses.
变形链球菌是龋齿的主要病因，传统牙膏、刷牙、使用牙线或抗菌漱口水都无法对其进行治疗。因此，有必要开发强化牙膏。蓝藻来源的藻红蛋白（PE）具有抗氧化和抗菌特性。磁场（MF）处理对蓝藻的新陈代谢和抗氧化防御也有效果。本研究旨在开发含有嗜鱼蓝细菌属色素以及油柑叶油的牙膏，并在30和60毫特斯拉（mT）的磁场下评估其抗氧化和抗菌性能。对5种牙膏进行了45天的多项评估，结果表明，30 mT的磁场具有最高的波长和PE吸收率。此外，与对照组相比，经磁场处理的培养物中PE的纯度大幅提高。牙膏处理对变形链球菌的生长抑制区平均直径的研究结果表明，与对照处理相比，油柑叶油和PE的组合使抗菌活性大幅提高（1.5倍）（p<0.05）。与对照相比，在磁场下添加PE和油柑叶油可减少水分流失（1.5倍），增强质地均匀性（2倍），增加酚类含量（2倍），提高抗氧化活性，并降低牙膏处理的pH值（p<0.05）。此外，对各种牙膏处理的总体可接受性进行感官评估的结果表明，与对照处理相比，油柑叶油和PE的组合提高了牙膏处理的总体可接受性（p<0.05）。总体而言，在牙膏配方中使用磁场下的PE和油柑叶油对减少龋齿和牙周疾病特别有益。
