Paredes S R, Kozicki P A, Batlle A M
Comp Biochem Physiol B. 1985;82(4):751-7. doi: 10.1016/0305-0491(85)90520-6.
The effect of S-adenosyl-L-methionine (SAM) administration to both acute and chronic lead exposed mice was investigated. SAM was given s.c. at different doses and for different time intervals. The best results were obtained using 20 mg SAM/kg applied daily over a period of 20-22 days. Results obtained in both acute and chronic lead poisoning were quite similar. GSH concentration in blood and liver, reduced in intoxicated animals was increased after SAM administration reaching normal values. Blood, liver and kidney lead content notably increased at the beginning of SAM treatment and decreased rapidly in the group receiving SAM, attaining values near control levels in 2 weeks. A significant recovery of blood, liver, kidney, spleen and brain delta-aminolevulic acid dehydratase (ALA-D) initially reduced in poisoned animals, was clearly produced after SAM administration. A clear and direct correlation between the recovery of both ALA-D activity and GSH levels and the decreased concentration of lead in tissues was observed, reinforcing our proposal that enhancement of thiol content as a result of SAM administration would facilitate the detoxification process and lead removal, consequently reversing the inactivation of the enzyme. We conclude that SAM therapy is beneficial in the treatment of lead intoxication.
研究了给予急性和慢性铅暴露小鼠S-腺苷-L-甲硫氨酸（SAM）的效果。SAM以不同剂量和不同时间间隔进行皮下注射。在20 - 22天的时间里每天给予20 mg SAM/kg可获得最佳结果。急性和慢性铅中毒所获得的结果非常相似。中毒动物血液和肝脏中降低的谷胱甘肽（GSH）浓度在给予SAM后升高至正常水平。在SAM治疗开始时，血液、肝脏和肾脏中的铅含量显著增加，而在接受SAM的组中迅速下降，在2周内达到接近对照水平的值。中毒动物中最初降低的血液、肝脏、肾脏、脾脏和脑δ-氨基-γ-酮戊酸脱水酶（ALA-D）在给予SAM后明显恢复。观察到ALA-D活性和GSH水平的恢复与组织中铅浓度降低之间存在明显的直接相关性，这加强了我们的观点，即给予SAM导致硫醇含量增加将促进解毒过程和铅的清除，从而逆转酶的失活。我们得出结论，SAM疗法对铅中毒的治疗有益。
