Yohannes Lamrot, Birhan Tsegaye Adane, Endalew Mastewal, Azanaw Jember, Weldegebrel Fasika
Department of Environmental and Occupational Health and Safety, Institute of Public Health, College of Medicine and Other Health Sciences, University of Gondar, Gondar, Ethiopia.
PLoS One. 2025 Sep 9;20(9):e0331546. doi: 10.1371/journal.pone.0331546. eCollection 2025.
Foodborne diseases pose a significant public health challenge worldwide. The increasing availability of edible oils in the market, combined with Ethiopia's lack of stringent quality control and regulatory oversight, raises concerns about their safety. This inadequacy in regulation may contribute to microbial contamination, leading to potential public health risks. A laboratory-based cross-sectional study was conducted from May to July 2021. Twelve samples of Niger seed oil (Guizotia abyssinica (Linnaeus f.) Cassini) were collected using a universal sampling technique. In the microbiology laboratory, the samples were aseptically examined for bacterial and fungal contamination using standard microbiological methods and procedures. The collected data were entered and analyzed using Stata Version 14. Mean values and standard deviations were computed, and the results were presented in text and tables. Microbial analysis of the 12 Niger seed oil (Guizotia abyssinica (Linnaeus f.) Cassini) samples revealed varying levels of bacterial, mold, and coliform contamination. The identified bacterial species included Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa, while Aspergillus niger, Aspergillus flavus, and Aspergillus fumigatus were the predominant fungal isolates. The total aerobic bacterial count ranged from 1.3 × 103 to 9.2 × 104 cfu/ml, with the highest recorded mold count reaching 4 × 10⁵ cfu/ml. Additionally, both total and fecal coliform isolates were detected in the samples. The presence of these microorganisms suggests that the oil processing, production, handling, and storage systems lack proper hygienic handling practices. This finding highlights the urgent need for stringent hygiene measures, enhanced quality control protocols, and strict adherence to food safety regulations throughout the production and distribution processes.
食源性疾病在全球范围内构成了重大的公共卫生挑战。市场上食用油供应的增加，再加上埃塞俄比亚缺乏严格的质量控制和监管监督，引发了对其安全性的担忧。这种监管不足可能导致微生物污染，从而带来潜在的公共卫生风险。2021年5月至7月进行了一项基于实验室的横断面研究。使用通用采样技术收集了12份黑籽油（Guizotia abyssinica (Linnaeus f.) Cassini）样本。在微生物实验室中，使用标准微生物学方法和程序对样本进行无菌检查，以检测细菌和真菌污染情况。收集的数据录入并使用Stata 14版本进行分析。计算了平均值和标准差，并以文字和表格形式呈现结果。对12份黑籽油（Guizotia abyssinica (Linnaeus f.) Cassini）样本的微生物分析显示，细菌、霉菌和大肠菌群污染程度各不相同。鉴定出的细菌种类包括金黄色葡萄球菌、肺炎克雷伯菌和铜绿假单胞菌，而黑曲霉、黄曲霉和烟曲霉是主要的真菌分离株。需氧细菌总数在1.3×10³至9.2×10⁴ cfu/ml之间，记录到的最高霉菌数达到4×10⁵ cfu/ml。此外，样本中还检测到了总大肠菌群和粪大肠菌群分离株。这些微生物的存在表明，油脂加工、生产、处理和储存系统缺乏适当的卫生处理措施。这一发现凸显了在整个生产和分销过程中迫切需要采取严格的卫生措施、加强质量控制方案并严格遵守食品安全法规。
