成年血管化猪视网膜外植体在纳米管支架上的体外器官型培养
Organotypic Culture of Adult Vascularized Porcine Retina Explants In Vitro on Nanotube Scaffolds.
作者信息
Friebe Sabrina, Weigel Solveig, Francke Mike, Mayr Stefan G
机构信息
Division of Surface Physics, Department of Physics and Earth System Sciences, University of Leipzig, Linnéstr. 5, 04103, Leipzig, Germany.
Department of Biocompatible and Bioactive Surfaces, Leibniz Institute of Surface Engineering (IOM), Permoserstr. 15, 04318, Leipzig, Germany.
出版信息
Biol Proced Online. 2025 Sep 8;27(1):35. doi: 10.1186/s12575-025-00301-5.
BACKGROUND
Organotypic long-term cultivation of vascularized retina explants is a major challenge for application in drug development, drug screening, diagnostics and future personalized medicine. With this background, an assay and protocol for organotypic culture of vascularized retina explants in vitro with optimum tissue integrity preservation is developed and demonstrated.
METHODS
Morphological, histologic and biochemical integrity as well as viability of vascularized retina explants are compared as function of cultivation time for differently structured nanotube scaffolds. In doing so, porcine retina explants obtained from a local slaughterhouse are employed as paradigm for vascularized retina.
CONCLUSIONS
We demonstrate that titania nanotube arrays are highly promising as culturing scaffold of vascularized retina explants in vitro due to highly tunable surface properties regarding biomedical signaling. The unprecedented maintenance of tissue integrity allows for screening of pharmacological drugs and disease mechanisms in an ex-vivo test-based culture system with reduced need for animal experiments.
背景
血管化视网膜外植体的器官型长期培养是药物开发、药物筛选、诊断及未来个性化医疗应用中的一项重大挑战。在此背景下，我们开发并展示了一种用于体外器官型培养血管化视网膜外植体的检测方法和方案，该方案能最佳程度地保留组织完整性。
方法
将不同结构的纳米管支架培养时间作为函数，比较血管化视网膜外植体的形态学、组织学和生化完整性以及活力。在此过程中，采用从当地屠宰场获取的猪视网膜外植体作为血管化视网膜的范例。
结论
我们证明，由于二氧化钛纳米管阵列在生物医学信号传导方面具有高度可调的表面特性，因此作为体外血管化视网膜外植体的培养支架极具前景。前所未有的组织完整性维持使得在基于体外试验的培养系统中筛选药理药物和疾病机制成为可能，同时减少了动物实验的需求。
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