de Sousa Alessandra Tammy Hayakawa Ito, Makino Herica, Costa Marco Túlio Dos Santos, Cândido Stefano Luis, Gomes Kaio Lierlyson Teles, Chitarra Cristiane Silva, Pepato Marco Andrey, de Azevedo Francisco Kennedy Scofoni Faleiros, Souto Francisco Jose Dutra, de Almeida Arleana Bom Parto Ferreira, Sousa Valeria Régia Franco, Nakazato Luciano, Dutra Valéria
Microbiology Laboratory, Veterinary Hospital of the Federal University of Mato Gross - UFMT, Cuiabá, Mato Grosso, Brazil.
Department of Clinical Medicine, Júlio Muller University Hospital of the Federal University of Mato Grosso - UFMT, Cuiabá, Mato Grosso, Brazil.
Vet World. 2025 Jul;18(7):2012-2023. doi: 10.14202/vetworld.2025.2012-2023. Epub 2025 Jul 22.
The global rise of multidrug-resistant (MDR) poses a serious threat to human and animal health. Close proximity between humans and domestic animals may facilitate zoonotic transmission of MDR strains, underscoring the need for integrated surveillance strategies. This study aimed to investigate the genetic diversity, resistance mechanisms, and virulence gene profiles of isolates from domestic animals and humans in Mato Grosso, Brazil, within the One Health framework.
A total of 48 clinical isolates (33 from animals and 15 from humans) were analyzed. Identification was confirmed through 16S ribosomal RNA sequencing. Antimicrobial susceptibility was tested using disk diffusion (animal isolates) and minimum inhibitory concentration (human isolates). Resistance ( and ) and virulence genes (, , , , etc.) were detected through polymerase chain reaction. Multilocus sequence typing (MLST) was performed on seven housekeeping genes, and sequence types (STs) were assigned using the Pasteur Institute database (Paris, France).
MDR phenotypes were found in 70.83% (34/48) of isolates - 78.78% of animal and 53% of human samples. Virulence genes were present in 77.08% of isolates; was the most prevalent (60.61%). The gene was found in three human isolates, and was found in one human and one bovine isolate. MLST revealed 39 STs, including 9 novel ones. Clonal complexes (CC)258 (human), CC15 (animal), and CC147 (both species) indicated potential interspecies transmission.
This study provides the first comprehensive molecular epidemiological snapshot of in domestic animals and humans in Mato Grosso. The discovery of shared clonal complexes and high MDR rates demands urgent cross-sectoral surveillance and control strategies under the One Health approach.
多重耐药菌（MDR）在全球范围内的增多对人类和动物健康构成了严重威胁。人类与家畜的密切接触可能会促进MDR菌株的人畜共患病传播，这凸显了综合监测策略的必要性。本研究旨在在“同一健康”框架下，调查巴西马托格罗索州家畜和人类分离株的遗传多样性、耐药机制和毒力基因谱。
共分析了48株临床分离株（33株来自动物，15株来自人类）。通过16S核糖体RNA测序进行鉴定。使用纸片扩散法（动物分离株）和最低抑菌浓度法（人类分离株）检测抗菌药物敏感性。通过聚合酶链反应检测耐药基因（blaTEM、blaSHV等）和毒力基因（mecA、tst、eta、etb等）。对7个管家基因进行多位点序列分型（MLST），并使用法国巴黎巴斯德研究所数据库确定序列类型（STs）。
70.83%（34/48）的分离株呈现MDR表型——78.78%的动物样本和53%的人类样本。77.08%的分离株存在毒力基因；mecA最为常见（60.61%）。在3株人类分离株中发现了blaNDM基因，在1株人类和1株牛分离株中发现了blaVIM基因。MLST显示有39种STs，包括9种新的STs。克隆复合体（CC）258（人类）、CC15（动物）和CC147（两种物种）表明存在潜在的种间传播。
本研究提供了巴西马托格罗索州家畜和人类中葡萄球菌的首个全面分子流行病学概况。共享克隆复合体的发现和高MDR率要求在“同一健康”方法下实施紧急的跨部门监测和控制策略。
