Zaini Lia Meuthia, Kartasasmita Arief S, Gondhowiardjo Tjahjono D, Syukri Maimun, Lesmana Ronny
Faculty of Medicine, Department of Medical Education, Syiah Kuala University, Banda Aceh, Indonesia.
Department of Ophthalmology, Zainoel Abidin Hospital, Banda Aceh, Indonesia.
J Ophthalmic Vis Res. 2025 Sep 2;20. doi: 10.18502/jovr.v20.15231. eCollection 2025.
To assess the effect of empagliflozin on the expression of SGLT-2 and GLUT-1 in the chorioretina of streptozotocin-induced diabetic rats.
An experimental study was performed on Wistar rats. After a 2-week adaptation period, the rats were allocated to one of four groups. The group allocations were as follows: (K1) negative control (no intervention); (K2) positive control injected with streptozotocin (STZ) without treatment; (K3) positive control injected with STZ and treated with metformin; and (K4) positive control injected with STZ and treated with empagliflozin. SGLT-2 and GLUT-1 expressions were examined using the Western blot technique.
SGLT-2 was expressed in the chorioretinal tissue of all groups; administration of SGLT-2 inhibitors could suppress its expression. The average band thickness indicated that SGLT-2 expression was higher in diabetic rats compared to rats with normal sugar levels. GLUT-1 expression was noticed in all groups based on the chorioretinal examination. It did not change in the metformin group, whereas the empagliflozin group showed a significant decrease in the GLUT-1 expression.
Both metformin and empagliflozin suppress the expression of SGLT-2 and GLUT-1 in the chorioretina of diabetic rats. In comparison, empagliflozin seems to be more potent in reducing this expression.
评估恩格列净对链脲佐菌素诱导的糖尿病大鼠脉络膜视网膜中钠-葡萄糖协同转运蛋白2（SGLT-2）和葡萄糖转运蛋白1（GLUT-1）表达的影响。
对Wistar大鼠进行实验研究。经过2周的适应期后，将大鼠分为四组之一。分组情况如下：（K1）阴性对照（无干预）；（K2）注射链脲佐菌素（STZ）的阳性对照，未治疗；（K3）注射STZ并接受二甲双胍治疗的阳性对照；（K4）注射STZ并接受恩格列净治疗的阳性对照。采用蛋白质印迹技术检测SGLT-2和GLUT-1的表达。
所有组的脉络膜视网膜组织中均表达SGLT-2；给予SGLT-2抑制剂可抑制其表达。平均条带厚度表明，糖尿病大鼠中SGLT-2的表达高于血糖正常的大鼠。基于脉络膜视网膜检查，所有组均观察到GLUT-1的表达。二甲双胍组GLUT-1表达未改变，而恩格列净组GLUT-1表达显著降低。
二甲双胍和恩格列净均可抑制糖尿病大鼠脉络膜视网膜中SGLT-2和GLUT-1的表达。相比之下，恩格列净在降低这种表达方面似乎更有效。
