Shi Yaohong, Saito Yutaro, Hotta Masashi, Akita Mayuko, Uwamizu Akiharu, Aoki Junken, Kunisawa Jun, Sando Shinsuke
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Tokyo 113-8656 Japan
Laboratory of Vaccine Materials, Laboratory of Gut Environmental System, Microbial Research Center for Health and Medicine, National Institutes of Biomedical Innovation, Health and Nutrition (NIBN) 7-6-8 Asagi Saito Osaka 567-0085 Ibaraki Japan.
RSC Adv. 2025 Sep 8;15(39):32263-32270. doi: 10.1039/d5ra04836b. eCollection 2025 Sep 5.
Polyunsaturated fatty acids (PUFAs), fatty acids with multiple unsaturated carbon-carbon bonds, constitute a crucial class of lipids. While the vast diversity of PUFA species arises from their structural variations, most of them are poorly investigated due to their limited availability. Here, we utilize solid-phase synthesis of PUFAs, which we have recently developed, to construct a PUFA library. With the library, we investigate agonist activities for free fatty acid receptors 1 (FFAR1) and 4 (FFAR4), inhibition abilities against neutrophil pseudopod formation, and anti-inflammatory effects on the hypersensitivity mouse model. This study highlights the utility of PUFA solid-phase synthesis as a powerful approach for elucidating the relationship between the chemical structures of PUFAs and their biological functions.
多不饱和脂肪酸（PUFAs）是一类具有多个不饱和碳 - 碳双键的脂肪酸，是脂质的重要组成部分。虽然PUFA种类繁多，源于其结构差异，但由于其可得性有限，大多数PUFA的研究较少。在此，我们利用我们最近开发的PUFA固相合成方法构建了一个PUFA文库。利用该文库，我们研究了对游离脂肪酸受体1（FFAR1）和4（FFAR4）的激动剂活性、对中性粒细胞伪足形成的抑制能力以及对超敏反应小鼠模型的抗炎作用。本研究突出了PUFA固相合成作为一种强大方法在阐明PUFA化学结构与其生物学功能之间关系方面的实用性。
