在各种条件下分离得到的肾基底膜的组成。

Composition of renal basement membranes isolated under various conditions.

作者信息

Langeveld J P, de Wit F, Reubsaet F A, Monnens L A, van Haelst U J, de Boer H, Veerkamp J H

出版信息

Int J Biochem. 1985;17(12):1373-81. doi: 10.1016/0020-711x(85)90063-1.

Abstract

GBM isolated from a surgical biopsy directly or after a 22 hr incubation period--to imitate the usual interval between death and isolation--appeared to be nearly identical in amino acid composition. Sonication and detergent procedures for isolation of GBM and TBM lead to preparations of different chemical composition. Phosphorus analysis and electron micrographs indicate the presence of material of supposedly cellular origin in sonicated but not in detergent-treated bovine and human GBM. Detergent-treated bovine and human GBM preparations are more enriched in the collagen-typical amino acids than sonicated samples. SDS-PAGE analyses show a nearly identical polypeptide pattern. Sonicated and detergent-treated bovine TBM preparations are free of cellular material. They show in SDS-PAGE a similar heterogeneous polypeptide pattern, but with lower intensities of three components with molecular weights between 30 and 60 kdalton. Sulfated GAG's are present in higher concentration in sonicated than in detergent-treated GBM and TBM. Collagen is not extracted from glomeruli and tubules by detergent treatment.

摘要

直接从手术活检中分离得到的肾小球基底膜（GBM），或经过22小时孵育期（以模拟死亡与分离之间的通常间隔）后分离得到的GBM，其氨基酸组成似乎几乎相同。用于分离GBM和肾小管基底膜（TBM）的超声处理和去污剂处理程序会导致化学成分不同的制剂。磷分析和电子显微镜照片表明，在超声处理的牛和人GBM中存在推测为细胞来源的物质，而在经去污剂处理的GBM中则不存在。经去污剂处理的牛和人GBM制剂比超声处理的样品富含更多典型的胶原蛋白氨基酸。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析显示出几乎相同的多肽图谱。超声处理和经去污剂处理的牛TBM制剂不含细胞物质。它们在SDS-PAGE中显示出相似的异质多肽图谱，但分子量在30至60千道尔顿之间的三种成分强度较低。硫酸化糖胺聚糖（GAG）在超声处理的GBM和TBM中的浓度高于经去污剂处理的GBM和TBM。通过去污剂处理不能从肾小球和肾小管中提取胶原蛋白。