Zili Wang, Weidong Jia, Yu Zhang, Bing Xia, Liangkui Wei, Jianfeng Li, Fuyun Liu
Department of Orthopedics, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Spine Surgery, Zhumadian Central Hospital, Zhumadian, China.
Childs Nerv Syst. 2025 Sep 10;41(1):274. doi: 10.1007/s00381-025-06935-w.
To analyze the filum terminale (FT) of children with tethered cord syndrome (TCS) and aborted fetuses without neurological disorders in order to investigate the expression of significantly differentially expressed proteins in the FT under both pathological and physiological conditions.
According to the inclusion and exclusion criteria, 35 FT samples were selected, and the samples were subjected to immunohistochemistry and H&E staining. The data were analyzed using one-way analysis of variance, and P < 0.05 was considered statistically significant.
One out of 7 FTs in the aborted fetus group had fibrotic changes, 10 out of 14 FTs in the tethered cord (TC) group had fibrotic changes, and 14 out of 14 FTs in the TCS group had fibrotic changes. Most of the elastic fibers were missing and the degree of fibrotic changes was most severe in the TCS group, while the FTs in the TC group and the fetal group mainly showed only mild fibrosis. There were 10 cases (71.4%) showing strong expression of glutathione-S-transferase Pi 1 (GSTP1) in the TCS group, 8 cases (57.1%) of GSTP1 expression in children with TC, and 1 case (14.3%) of GSTP1 expression in the aborted fetus group.
In the TCS group, the FTs showed fibrotic changes, disorder, and increased adipose tissue, and most of the lesions were considered severe. The expression of GSTP1 was highest in the TCS group.
分析脊髓拴系综合征（TCS）患儿及无神经系统疾病的流产胎儿的终丝（FT），以研究在病理和生理条件下FT中显著差异表达蛋白的表达情况。
根据纳入和排除标准，选取35份FT样本，对样本进行免疫组织化学和苏木精-伊红染色。采用单因素方差分析对数据进行分析，P<0.05认为差异有统计学意义。
流产胎儿组7份FT中有1份出现纤维化改变，脊髓拴系（TC）组14份FT中有10份出现纤维化改变，TCS组14份FT均出现纤维化改变。TCS组大部分弹性纤维缺失，纤维化改变程度最严重，而TC组和胎儿组的FT主要仅表现为轻度纤维化。TCS组有10例（71.4%）谷胱甘肽-S-转移酶Pi 1（GSTP1）呈强表达，TC患儿中有8例（57.1%）GSTP1表达，流产胎儿组有1例（14.3%）GSTP1表达。
TCS组FT表现为纤维化改变、结构紊乱及脂肪组织增多，多数病变较严重。TCS组GSTP1表达最高。
