Timms L L, Schultz L H
J Dairy Sci. 1985 Dec;68(12):3363-6. doi: 10.3168/jds.S0022-0302(85)81248-0.
N-Acetyl-B-D-glucosaminidase activity, somatic cell count, and udder infection status were determined in milk of nine Saanen goats. Plasma enzyme activity was also measured. Individual half udder milk samples were taken for 12 d over a 3-wk period and animals were bled weekly. Three of the 18 udder halves were infected with coagulase-negative staphylococci over all 12 sampling d. The N-acetyl-B-D-glucosaminidase and somatic cells in milk were significantly elevated in samples where minor pathogens were isolated. Plasma enzyme was variable among goats but not within goats or across weeks. Greater daily variation was seen in somatic cell count as compared to milk enzyme activity. Correlation between milk N-acetyl-B-D-glucosaminidase and somatic cell count was .54.
测定了9只萨能山羊乳汁中的N-乙酰-β-D-氨基葡萄糖苷酶活性、体细胞计数和乳房感染状况。还测量了血浆酶活性。在3周的时间内,每隔12天采集一次单个半侧乳房的乳汁样本,每周对动物进行采血。在所有12个采样日中,18个半侧乳房中有3个感染了凝固酶阴性葡萄球菌。在分离出少量病原体的样本中,乳汁中的N-乙酰-β-D-氨基葡萄糖苷酶和体细胞显著升高。山羊之间血浆酶存在差异,但在同一只山羊内或不同周之间无差异。与乳汁酶活性相比,体细胞计数的每日变化更大。乳汁N-乙酰-β-D-氨基葡萄糖苷酶与体细胞计数之间的相关性为0.54。