Wang Beibei, Zhang Dandan, Zhou Gang, Li Xiaodong, Sun Tingli, Shi Qingshan, Xie Xiaobao
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
School of Life Sciences, Guangzhou University, Guangzhou 510006, China.
Nanomaterials (Basel). 2025 Aug 22;15(17):1296. doi: 10.3390/nano15171296.
Combating antibiotic resistance is critically significant for global public health. The development of new antibacterial nanomaterial is a promising way to do this. In this study, a bottom-up approach was employed to fabricate antibacterial carbon dots (ACDs). During the synthesis, quaternary ammonium function groups with long alkyl chains were successfully grafted on ACDs' surfaces. The obtained ACDs exhibited potent inhibitory against methicillin-resistant (MRSA) bacteria with minimum inhibitory concentrations of 2.5 µg/mL. Crucially, 2.5 µg/mL of ACDs could inhibit the growth of MRSA for as long as 72 h, which highlighted their long-term activity. Mechanistic investigations revealed that ACDs exerted bactericidal effects for MRSA bacteria primarily through disrupting the cell wall/membrane, destroying cell membrane potential, inducing the generation of excessive ROS, and triggering the leakage of nucleic acids and intracellular components. In sum, this work provided a kind of ACD with high efficiency and long-term antibacterial activity, offering promising potential for combating drug-resistant bacterial infections.
对抗抗生素耐药性对全球公共卫生至关重要。开发新型抗菌纳米材料是实现这一目标的一种有前途的方法。在本研究中，采用自下而上的方法制备了抗菌碳点（ACD）。在合成过程中，成功地将带有长烷基链的季铵官能团接枝到ACD表面。所获得的ACD对耐甲氧西林金黄色葡萄球菌（MRSA）表现出强效抑制作用，最低抑菌浓度为2.5 µg/mL。至关重要的是，2.5 µg/mL的ACD能够抑制MRSA生长长达72小时，这突出了它们的长期活性。机理研究表明，ACD对MRSA细菌发挥杀菌作用主要是通过破坏细胞壁/细胞膜、破坏细胞膜电位、诱导过量活性氧的产生以及引发核酸和细胞内成分的泄漏。总之，这项工作提供了一种具有高效和长期抗菌活性的ACD，为对抗耐药细菌感染提供了有前景的潜力。
