Zhang Huijie, Saadeldin Mohamed G, Buesen Darren, Elfaitory Hamzah, Burger Jakob, Friebe Vincent M, Honacker Jonas, Vöpel Tobias, Oughli Alaa A, Plumeré Nicolas
School of New Energy, Nanjing University of Science and Technology, Jiangyin, Jiangsu 214443, China.
Professorship for Electrobiotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstraße 53, 94315 Straubing, Germany.
Sci Adv. 2025 Sep 12;11(37):eadw6133. doi: 10.1126/sciadv.adw6133.
Oxidase-based electrochemical biosensors are widely deployed for point-of-use applications, yet oxygen interference remains a major challenge, substantially reducing sensing accuracy. Here, we developed a universal enzymatic O scavenger composed of alcohol oxidase, catalase, and paraformaldehyde to eliminate O within the sensor by converting it to water. Unlike other oxidases, alcohol oxidase exclusively uses O as an electron acceptor, preventing interference with the electron transfer chain involving the sensing oxidase. We demonstrated the compatibility of this O scavenger for calibration-free sensing of glucose, lactate, and creatinine in the concentration range relevant to human health. Without the O scavenger, sensor readings were less than 50% of those under inert gas conditions. With the O scavenger, the accuracy improved to 99%, even at low substrate concentrations. The general compatibility and performances of this alcohol oxidase-based O scavenger unlock the full potential of oxidase-based biosensors for reliable point-of-use sensing.
基于氧化酶的电化学生物传感器广泛应用于即时检测，但氧气干扰仍是一个重大挑战，严重降低了传感精度。在此，我们开发了一种由醇氧化酶、过氧化氢酶和多聚甲醛组成的通用酶促氧气清除剂，通过将传感器内的氧气转化为水来消除氧气。与其他氧化酶不同，醇氧化酶仅将氧气用作电子受体，从而避免干扰涉及传感氧化酶的电子传递链。我们证明了这种氧气清除剂与用于人体健康相关浓度范围内葡萄糖、乳酸和肌酐的免校准传感具有兼容性。没有氧气清除剂时，传感器读数不到惰性气体条件下读数的50%。有了氧气清除剂，即使在低底物浓度下，准确度也提高到了99%。这种基于醇氧化酶的氧气清除剂的普遍兼容性和性能释放了基于氧化酶的生物传感器用于可靠即时检测的全部潜力。
