Miyoshi Y, Kilpatrick K A, Kelley R O, Searles R P, Williams R C
J Rheumatol. 1985 Dec;12(6):1097-104.
IgG and IgM antilymphocyte antibodies were determined in sera from patients with systemic lupus erythematosus (SLE) by an ELISA method using isolated lymphocyte plasma membranes as antigens. Lymphocyte membranes prepared from T or B cell lines or normal peripheral blood showed a high degree of purity by enzyme assay and electron microscopic monitoring. Sera from patients with active SLE showed high IgG and IgM ELISA reactivity against T and B cell plasma membranes compared with normal sera. IgM antibodies to lymphocyte membranes showed a correlation with microcytotoxicity results. Serial studies showed a relationship of antimembrane antibody to disease activity in some patients, but not in others. The ELISA method for antilymphocyte antibody assay provides a sensitive technique for monitoring reactivity against antigens retained in isolated lymphocyte membrane preparations.
采用酶联免疫吸附测定（ELISA）法，以分离的淋巴细胞质膜作为抗原，测定系统性红斑狼疮（SLE）患者血清中的IgG和IgM抗淋巴细胞抗体。通过酶分析和电子显微镜监测，由T或B细胞系或正常外周血制备的淋巴细胞膜显示出高度的纯度。与正常血清相比，活动性SLE患者的血清对T和B细胞质膜显示出较高的IgG和IgM ELISA反应性。针对淋巴细胞膜的IgM抗体与微量细胞毒性结果相关。系列研究表明，在一些患者中抗膜抗体与疾病活动有关，但在另一些患者中则不然。用于抗淋巴细胞抗体测定的ELISA方法为监测针对分离的淋巴细胞膜制剂中保留的抗原的反应性提供了一种灵敏的技术。
