Hu Feiyue, Qin Jing, Yang Haosen, Al-Dhabi Naif Abdullah, Dong Yongen, Bai Zhixuan, Liu Lanhua, Chai Fengguang, Jin Baodan, Tang Wangwang, Ji Jiantao
School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, China.
Addiriyah Chair for Environmental Studies, Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
Bioresour Technol. 2026 Jan;439:133311. doi: 10.1016/j.biortech.2025.133311. Epub 2025 Sep 10.
Hydroxylamine supplementation has recently emerged as a potential strategy to persistent sustain partial nitration (PN), but its feasibility in the biofilm system of anammox/endogenous partial denitrification combined with continuous-flow partial nitration (SAEPD-CFPN) systems remains unexplored. Thus, this study assessed the feasibility of hydroxylamine dosing for restoring PN in continuous-flow biofilm reactor and investigated the impact of continuous-flow partial nitration (CFPN) deterioration on biofilm system of SAEPD-CFPN. Results showed that nitrogen removal efficiency (NRE) decreased from 88.86 % to 39.56 %, with the nitrite accumulation rate (NAR) dropping from 87.28 % to 34.33 % due to the deterioration of CFPN. After effective hydroxylamine dosing, the average NRE increased to 89.90 %, while the average NAR rose to 83.83 %. The SAEPD biofilm system could handle insufficient NO-N supply caused by CFPN performance insignificant deterioration; however, its effectiveness diminished when CFPN performance severely deteriorated over the long term due to lower influent COD concentrations. Metagenomic analysis revealed that CFPN recovery was attributed to the effective inhibition of Nitrobacter and NxrAB. The robustness of the SAEPD biofilm reactor against fluctuations in influent NO-N was attributable to its complex microbial community structure. Additionally, intermittent hydroxylamine dosing was proposed as a sustainable strategy to ensure stable SAEPD-CFPN biofilm process operation.
羟胺补充最近已成为一种维持部分硝化（PN）的潜在策略，但它在厌氧氨氧化/内源性部分反硝化与连续流部分硝化（SAEPD-CFPN）系统的生物膜系统中的可行性仍未得到探索。因此，本研究评估了在连续流生物膜反应器中添加羟胺恢复PN的可行性，并研究了连续流部分硝化（CFPN）恶化对SAEPD-CFPN生物膜系统的影响。结果表明，由于CFPN恶化，氮去除效率（NRE）从88.86%降至39.56%，亚硝酸盐积累率（NAR）从87.28%降至34.33%。有效添加羟胺后，平均NRE提高到89.90%，而平均NAR升至83.83%。SAEPD生物膜系统可以应对CFPN性能轻微恶化导致的NO-N供应不足；然而，当CFPN性能因进水COD浓度降低而长期严重恶化时，其有效性会降低。宏基因组分析表明，CFPN的恢复归因于对硝化杆菌和NxrAB的有效抑制。SAEPD生物膜反应器对进水NO-N波动的稳健性归因于其复杂的微生物群落结构。此外，提出间歇性添加羟胺作为一种可持续策略，以确保SAEPD-CFPN生物膜工艺稳定运行。
