Chang Longyan, Ning Dingying, Nan Fuxiong, Yang Wenshuo, Fan Yile, Tian Wenqing, Li Mingyue, Ying Jianjiang, Wu Di, Liang Jidong, Yan Wei
Department of Environmental Science and Engineering, Xi'an Jiaotong University, Xi'an 710035, China; Qianjiang Water Resources Development Co., Ltd., Hangzhou 310000, China.
Department of Environmental Science and Engineering, Xi'an Jiaotong University, Xi'an 710035, China; Green Architecture & Municipal Division, China Coal Xi'an Design Engineering Co., Ltd., Xi'an 710000, China.
Bioresour Technol. 2026 Jan;439:133304. doi: 10.1016/j.biortech.2025.133304. Epub 2025 Sep 10.
The partial denitrification coupled with Anammox (PDN/A) process enables energy-efficient nitrogen removal by overcoming nitrite and nitrate imbalance. This study compared the performance of PDN/A in a biofilter (BF) and a continuous stirred tank reactor (CSTR), focusing on nitrogen removal rates, microbial activity, and system stability. PDN/A-BF achieved a higher nitrogen removal rate (NRR) of 1.36 ± 0.12 kg-N m d compared to 0.67 ± 0.05 kg-N m d for PDN/A-CSTR. The PDN/A-BF system demonstrated superior Anammox nitrogen removal activity, with contributions of 91.09 ± 10.66 %, while PDN/A-CSTR achieved 72.42 ± 10.09 %. PDN/A-CSTR exhibited better stability under ultra-low substrate conditions, with a TN removal efficiency (TNRE) of 64.8 ± 3.41 % and COD removal efficiency (CODRE) of 58.88 ± 5.51 %. Dominant AnAOB genera included Ca. Brocadia in PDN/A-BF and Ca. Kuenenia in PDN/A-CSTR, with Pseudomonas and Thauera contributing to NO-N accumulation. PDN/A-BF outperformed PDN/A-CSTR in nitrogen removal efficiency but faced challenges like clogging and biofilm sloughing, and limiting long-term operation. PDN/A-CSTR, while less efficient, showed stable performance under low substrate conditions, making it suitable for low-strength wastewater treatment. Future research should focus on optimizing BF reactor design and exploring moving bio-carriers for CSTR to enhance AnAOB activity and system stability. These findings provide valuable insights for the engineering application of PDN/A in wastewater treatment.
部分反硝化耦合厌氧氨氧化（PDN/A）工艺通过克服亚硝酸盐和硝酸盐失衡实现了高效节能的氮去除。本研究比较了生物滤池（BF）和连续搅拌釜式反应器（CSTR）中PDN/A的性能，重点关注氮去除率、微生物活性和系统稳定性。与PDN/A-CSTR的0.67±0.05 kg-N m⁻³ d⁻¹相比，PDN/A-BF实现了更高的氮去除率（NRR），为1.36±0.12 kg-N m⁻³ d⁻¹。PDN/A-BF系统表现出卓越的厌氧氨氧化氮去除活性，贡献率为91.09±10.66%，而PDN/A-CSTR为72.42±10.09%。PDN/A-CSTR在超低底物条件下表现出更好的稳定性，总氮去除效率（TNRE）为64.8±3.41%，化学需氧量去除效率（CODRE）为58.88±5.51%。主要的厌氧氨氧化菌属在PDN/A-BF中为“Ca. Brocadia”，在PDN/A-CSTR中为“Ca. Kuenenia”，假单胞菌属和陶厄氏菌属导致了亚硝酸盐氮的积累。PDN/A-BF在氮去除效率方面优于PDN/A-CSTR，但面临堵塞和生物膜脱落等挑战，限制了长期运行。PDN/A-CSTR虽然效率较低，但在低底物条件下表现出稳定的性能，适用于低强度废水处理。未来的研究应专注于优化BF反应器设计，并探索用于CSTR的移动生物载体，以增强厌氧氨氧化菌活性和系统稳定性。这些发现为PDN/A在废水处理中的工程应用提供了有价值的见解。
