Noufeu Tchouli, Ming Tinghong, Zeng Xiaoqun, Xu Jiajie, Ngaba Mbezele Junior Yannick, Tchoumbougnang François, Pandong Achille Njomoue, Mpondo Salle, Keyeya Tchoulabi, Gouife Moussa
Microbial Development and Metabolic Engineering Laboratory, School of Marine Science, Ningbo University, Ningbo 315211, China.
State Key Laboratory for Quality and Safety of Agro-Products, College of Food Science and Engineering, Ningbo University, Ningbo 315211, China.
Foods. 2025 Aug 28;14(17):3009. doi: 10.3390/foods14173009.
() is globally recognized for its high nutritional value and potential as a sustainable food source. However, the influence of targeted nutrient supplementation on its biochemical composition and microbial safety under tropical open-pond conditions remains underexplored, particularly in sub-Saharan Africa. This study evaluated the effects of three nutrient supplementation regimes (compositions A, B, and C) and a control on cultivated over 30 days in raceway ponds at the Nomayos Spirulina Production Farm in Cameroon. All treatments maintained physicochemical parameters within ranges favorable for growth. Composition A significantly enhanced protein content (60.38 ± 0.68%), while composition C promoted carbohydrate accumulation (28.02 ± 0.41%). Microbial assessments revealed variable contamination levels, with composition B exhibiting the highest (1.05 ± 0.075 × 10 CFU/g) and (4.09 ± 1.81 × 10 CFU/g) counts, potentially due to nutrient-induced changes or post-harvest handling factors. Correlation analyses revealed a moderate positive relationship between nitrogen input and protein synthesis (r = 0.309), which was not statistically significant ( = 0.329). Additionally, higher pH was significantly correlated with total mesophilic counts (r = 0.661, = 0.019) and (r = 0.655, = 0.020). These findings highlight the importance of nutrient formulation and environmental management in improving nutritional quality while minimizing microbial risks during cultivation in tropical, low-tech settings.
（某事物）因其高营养价值和作为可持续食物来源的潜力而在全球范围内得到认可。然而，在热带露天池塘条件下，针对性营养补充对其生化成分和微生物安全性的影响仍未得到充分研究，特别是在撒哈拉以南非洲地区。本研究评估了三种营养补充方案（成分A、B和C）以及一个对照在喀麦隆诺马约斯螺旋藻生产农场的跑道池塘中培养30多天的效果。所有处理均将理化参数维持在有利于（某事物）生长的范围内。成分A显著提高了蛋白质含量（60.38±0.68%），而成分C促进了碳水化合物积累（28.02±0.41%）。微生物评估显示污染水平各不相同，成分B的（某两种微生物）计数最高（1.05±0.075×10 CFU/g）和（另一种微生物）计数最高（4.09±1.81×10 CFU/g），这可能是由于营养诱导的变化或收获后处理因素所致。相关性分析显示氮输入与蛋白质合成之间存在中等程度的正相关（r = 0.309），但无统计学意义（p = 0.329）。此外，较高的pH值与总嗜温菌计数（r = 0.661，p = 0.019）和（另一种微生物）计数（r = 0.655，p = 0.020）显著相关。这些发现凸显了在热带低技术环境下进行（某事物）培养时，营养配方和环境管理对于提高营养质量同时降低微生物风险的重要性。
