Zhang Yaran, Zhang Qinglan, Gao Yaping, Xiao Yao, Wang Jinpeng, Yang Chunhong, Ju Zhihua, Wei Xiaochao, Wang Xiuge, Jiang Qiang, Huang Jinming
Key Laboratory of Livestock and Poultry Multi-omics of Ministry of Agriculture and Rural Affairs, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Shandong Technology Innovation Center of Dairy Cattle Breeding Industry, Jinan 250100, China.
Animals (Basel). 2025 Sep 5;15(17):2607. doi: 10.3390/ani15172607.
TARDBP mediates milk fat secretion in mice by binding to UG-rich sequences in the 3' untranslated region (UTR) of and mRNA and enhancing their mRNA stability. However, its role in bovine milk lipid metabolism remains unclear. To investigate this, we generated knockout (KO) MAC-T cells using CRISPR/Cas9 technology, quantified triacylglycerol (TAG) levels in both cells and culture supernatant, and examined the impact of on mRNA levels in MAC-T cells through transcriptome sequencing. We found that deletion of reduced TAG content in both MAC-T cells and the supernatant, as well as decreased mRNA levels of , , , , and . However, the expression of and was unaffected in bovine MAC-T cells. Sequence analysis further revealed TG-rich sequences within bovine and but not in , , , or and . These findings suggest that may regulate bovine lipid metabolism through a mechanism distinct from that described in mice. This study provides new insights into the molecular role of in bovine milk fat metabolism and establishes a foundation for understanding its contribution to dairy cattle breeding and milk quality improvement.
TARDBP通过与 和 mRNA的3'非翻译区（UTR）中富含UG的序列结合并增强其mRNA稳定性来介导小鼠乳脂肪分泌。然而，其在牛乳脂质代谢中的作用仍不清楚。为了研究这一点，我们使用CRISPR/Cas9技术生成了 基因敲除（KO）的MAC-T细胞，定量了细胞和培养上清液中的三酰甘油（TAG）水平，并通过转录组测序研究了 对MAC-T细胞中mRNA水平的影响。我们发现， 的缺失降低了MAC-T细胞和上清液中的TAG含量，以及 、 、 、 和 的mRNA水平。然而， 和 的表达在牛MAC-T细胞中未受影响。序列分析进一步揭示，牛 和 中有富含TG的序列，但 、 、 或 中没有。这些发现表明， 可能通过一种不同于小鼠中描述的机制来调节牛的脂质代谢。本研究为 在牛乳脂肪代谢中的分子作用提供了新的见解，并为理解其对奶牛育种和牛奶品质改善的贡献奠定了基础。
