Okazaki M, Furuya E, Kasahara T, Sakamoto K
Jpn J Pharmacol. 1985 Dec;39(4):503-14. doi: 10.1254/jjp.39.503.
Phagocytic activity as a function of the reticuloendothelial system (RES) has been studied in CCl4-induced liver injury by using the carbon clearance test. Liver damage in mice was induced by administration of 20% CCl4 in olive oil (p.o.). After a single administration of CCl4, significant increases in liver/body weight ratio, serum GOT and GPT levels, alpha, beta and gamma-globulins and BSP retention, and decreases in serum albumin, an activity of the hepaplastintest and the correct phagocytic activity, alpha value, were found. After 15 administrations of CCl4 (3 times a week), mild increases in serum GPT level and BSP retention and decreases in the activity of the hepaplastintest and both phagocytic indices, K and alpha values, were observed. However, zymosan treatment 3 days before sacrifice induced an increase in K value depressed by multiple administrations of CCl4. The depression of carbon uptake by Kupffer cells can be seen by light microscopy after multiple administrations of CCl4 compared with that of saline and olive oil. These findings indicate that the RES phagocytosis is suppressed more strongly in chronic liver injury by 15 CCl4 administrations than in acute injury by a single one, although the biochemical parameters indicating liver injury are shown to have an opposite tendency. A clear correlation between the alteration of RES activity and the degree of liver injury was not noted.
通过碳清除试验研究了吞噬活性作为网状内皮系统（RES）的一项功能在四氯化碳诱导的肝损伤中的情况。给小鼠经口灌胃20%四氯化碳橄榄油溶液诱导肝损伤。单次给予四氯化碳后，发现肝/体重比、血清谷草转氨酶（GOT）和谷丙转氨酶（GPT）水平、α、β和γ球蛋白以及磺溴酞钠（BSP）潴留显著升高，血清白蛋白、肝促凝血酶原激酶试验活性和正确的吞噬活性α值降低。每周3次给予四氯化碳，共15次后，观察到血清GPT水平和BSP潴留轻度升高，肝促凝血酶原激酶试验活性以及吞噬指数K值和α值降低。然而，在处死前3天用酵母聚糖处理可使多次给予四氯化碳后降低的K值升高。与生理盐水和橄榄油组相比，多次给予四氯化碳后，光镜下可见枯否细胞对碳的摄取减少。这些发现表明，尽管显示肝损伤的生化参数呈相反趋势，但15次给予四氯化碳导致的慢性肝损伤中RES吞噬作用的抑制比单次给予导致的急性损伤中更强烈。未观察到RES活性改变与肝损伤程度之间有明显相关性。
