Roy Chowdhury Moumita, Mehmet Mizgin, Mukherjee Jit, Debnath Anirban Jyoti, Ražná Katarína
Faculty of Agrobiology and Food Resources, Institute of Plant and Environmental Sciences, Slovak University of Agriculture in Nitra, Nitra 94976, Slovakia.
Faculty of Agriculture, Department of Field Crops, Ege University, Izmir 35100, Türkiye.
Molecules. 2025 Aug 24;30(17):3476. doi: 10.3390/molecules30173476.
Chitosan (CTS) is a biodegradable and biocompatible biopolymer derived from chitin. Thanks to its diverse biological activities and environmentally friendly nature, it has emerged as a promising agent in plant tissue culture. Recent studies have highlighted its role as a natural elicitor that can enhance plant growth, seed germination, and the biosynthesis of secondary metabolites in vitro. In plant tissue culture, it acts as a biotic elicitor, mimicking a pathogen attack and activating the pathogenesis-related proteins to induce secondary metabolite production. In vitro tissue culture is a scientifically meaningful and cost-effective approach to testing the elicitation mechanisms of various abiotic elicitors, including CTS. However, the methodology of CTS elicitation in plant tissue cultures is not straightforward or uniform due to the differences in the CTS origin, molecular weight, and degree of deacetylation, all of which directly affect solubility. This review summarizes the methodological approaches to the use of CTS in plant tissue culture elicitation and highlights specific features of these procedures.
壳聚糖（CTS）是一种源自几丁质的可生物降解且具有生物相容性的生物聚合物。由于其多样的生物活性和环境友好的特性，它已成为植物组织培养中有前景的试剂。最近的研究突出了其作为天然激发子的作用，能够在体外增强植物生长、种子萌发以及次生代谢产物的生物合成。在植物组织培养中，它作为生物激发子，模拟病原体攻击并激活病程相关蛋白以诱导次生代谢产物的产生。体外组织培养是一种测试包括CTS在内的各种非生物激发子激发机制的科学且经济高效的方法。然而，由于CTS的来源、分子量和脱乙酰度不同，所有这些都直接影响溶解度，因此在植物组织培养中使用CTS的激发方法并不直接或统一。本综述总结了在植物组织培养激发中使用CTS的方法，并突出了这些程序的具体特点。
