Peter Anca, Monea Manuel Brendon, Mihaly Cozmuta Anca, Nicula Camelia, Mihaly Cozmuta Leonard, Vosgan Zorica, Szakacs Zsolt, Drazic Goran, Magyari Klara, Muresan-Pop Marieta, Baia Lucian
Faculty of Sciences, Technical University of Cluj Napoca, Victoriei 76, 430072 Baia Mare, Romania.
National Institute of Chemistry, Hajdrihova 19, P.O. Box 660, SI-1001 Ljubljana, Slovenia.
Polymers (Basel). 2025 Aug 26;17(17):2311. doi: 10.3390/polym17172311.
The design and development of scaffolds play a crucial role in tissue engineering. In this regard, the study aims to establish the influence of porosity on the morpho-structural, physical-chemical, and biochemical characteristics of the polylactic acid (PLA) and/or polycaprolactone (PCL) scaffolds, in order to be considered candidates for tissue reconstruction. The results indicated that binary PLA-PCL and PCL matrices are more suitable than PLA, due to their higher crystallization degree, this contributing to the superior mechanical properties and lower network defects. The preponderance of molecular interactions decreases with porosity. Porosity induced a decrease in the degree of crystallization of PLA-PCL and an increase in water, glucose and blood components uptake by 188, 178, and 28%, respectively. The PLA-PCL scaffold was found to be more stable to lipase action than neat PLA as a result of the reduced enzyme access due to the higher crystallinity and thermodynamic stability of the hydrocarbon linear chain in PCL, which is higher than that of the side methyl group in PLA. growth increases with porosity and was more pronounced on the PLA-PCL matrix. All these results show that varying the porosity and composition of the polymer mixture leads to valuable materials with nutrient absorption capacity and biodegradability superior to neat PLA or PCL materials.
支架的设计与开发在组织工程中起着至关重要的作用。在这方面，本研究旨在确定孔隙率对聚乳酸（PLA）和/或聚己内酯（PCL）支架的形态结构、物理化学和生化特性的影响，以便将其视为组织重建的候选材料。结果表明，二元PLA-PCL和PCL基质比PLA更合适，因为它们具有更高的结晶度，这有助于提高机械性能并减少网络缺陷。分子间相互作用的优势随着孔隙率的增加而降低。孔隙率导致PLA-PCL的结晶度降低，水、葡萄糖和血液成分的摄取分别增加了188%、178%和28%。由于PCL中烃类线性链的结晶度和热力学稳定性较高，导致酶的可及性降低，因此发现PLA-PCL支架比纯PLA对脂肪酶作用更稳定，PCL中烃类线性链的结晶度和热力学稳定性高于PLA中的侧甲基。细胞生长随着孔隙率的增加而增加，在PLA-PCL基质上更为明显。所有这些结果表明，改变聚合物混合物的孔隙率和组成会产生具有优于纯PLA或PCL材料的营养吸收能力和生物降解性的有价值材料。
