Hou Fengyan, Dong Jianjun, Wang Xia, Deng Qiuyang, Crabbe M James C, Wang Zuobin
International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China.
Centre for Opto/Bio-Nano Measurement and Manufacturing, Zhongshan Institute of Changchun University of Science and Technology, Zhongshan 528437, China.
Sensors (Basel). 2025 Aug 23;25(17):5255. doi: 10.3390/s25175255.
Based on the photoconductive effect of photosensitive films, a designed light pattern was projected onto a hydrogenated amorphous silicon (a-Si:H) photosensitive chip to generate virtual light-induced electrodes for cellular electrical detection. To obtain high-quality cellular signals, this study aims to explore the effect of electrical excitation on a-Si:H photosensitive chip. Firstly, the electrochemical impedance spectroscopy (EIS) and volt-ampere characteristics of the a-Si:H photosensitive chip were characterized. EIS data were fitted to extract equivalent circuit models (ECMs) for both the chip and system. Then analog experiments were performed to verify the ECMs, and the results were consistent with the circuit simulation. Finally, applied alternating current (AC) or direct current (DC) signals to the chip and recorded the electrical signals of the cultured cardiomyocytes on the a-Si:H photosensitive chip. The results demonstrated that applying a high-frequency small AC signal to the chip reduced the background noise of the system by approximately 85.1%, and applying a DC bias increased the amplitude of the detection signal by approximately 142.7%. Consequently, the detection performance of the a-Si:H photosensitive chip for weak bioelectrical signals was significantly enhanced, advancing its applicability in cellular electrophysiological studies.
基于光敏薄膜的光电导效应，将设计好的光图案投射到氢化非晶硅（a-Si:H）光敏芯片上，以生成用于细胞电检测的虚拟光诱导电极。为了获得高质量的细胞信号，本研究旨在探索电激发对a-Si:H光敏芯片的影响。首先，对a-Si:H光敏芯片的电化学阻抗谱（EIS）和伏安特性进行了表征。对EIS数据进行拟合，以提取芯片和系统的等效电路模型（ECM）。然后进行模拟实验以验证ECM，结果与电路仿真一致。最后，向芯片施加交流（AC）或直流（DC）信号，并记录a-Si:H光敏芯片上培养的心肌细胞的电信号。结果表明，向芯片施加高频小交流信号可使系统的背景噪声降低约85.1%，施加直流偏置可使检测信号的幅度增加约142.7%。因此，a-Si:H光敏芯片对微弱生物电信号的检测性能得到显著提高，推动了其在细胞电生理研究中的应用。
