Li Qingchen, Feng Yuguo, Zhuang Siying, Kang Linman, Yang Yang
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China.
Int J Mol Sci. 2025 Aug 28;26(17):8363. doi: 10.3390/ijms26178363.
This study systematically investigated the decolorization efficacy and detoxification effect of crude laccase derived from yang1 on azo and triphenylmethane dyes. This research encompassed decolorization efficiencies for 15 dyes (7 azo dyes and 8 triphenylmethane dyes), time course decolorization kinetics, and detoxification assessment using rice () and wheat () seed germination as phytotoxicity indicators for both single-dye and mixed-dye systems. Molecular docking was employed to elucidate the laccase-dye interaction mechanisms. The results demonstrated that crude laccase from yang1 exhibited significant decolorization efficiency and effective detoxification capacity toward both azo dyes and triphenylmethane dyes. It also displayed considerable decolorization efficiency for mixtures of azo and triphenylmethane dyes (mixture of two types of dyes), along with strong detoxification capability against the phytotoxicity of mixed dyes. Crude laccase showed robust continuous batch decolorization capability for azo dyes Alpha-naphthol Orange (α-NO) and Mordant Blue 13 (MB13). Similarly, it achieved high continuous batch decolorization efficiency for triphenylmethane dyes (e.g., Cresol Red, Acid Green 50) while maintaining stable laccase activity throughout the decolorization process. Crude laccase demonstrated excellent reusability and sustainable degradation performance during the continuous batch decolorization. The decolorization of crude laccase could significantly reduce or completely eliminate the phytotoxicity of both single dyes and mixtures of two dyes (pairwise mixtures of different types of dyes, totaling 18 different combinations). The results of molecular docking between the laccase protein and structurally diverse dyes further elucidated the underlying causes and potential mechanisms for variations in the catalytic ability of laccase toward different structural dyes. In summary, crude laccase from yang1 possessed great application value and potential for efficiently degrading and detoxifying dye pollutants of different structural types.
本研究系统地研究了源自yang1的粗漆酶对偶氮染料和三苯甲烷染料的脱色效果及解毒作用。该研究涵盖了15种染料（7种偶氮染料和8种三苯甲烷染料）的脱色效率、时间进程脱色动力学，以及使用水稻（）和小麦（）种子发芽作为单染料和混合染料系统植物毒性指标的解毒评估。采用分子对接来阐明漆酶 - 染料相互作用机制。结果表明，源自yang1的粗漆酶对偶氮染料和三苯甲烷染料均表现出显著的脱色效率和有效的解毒能力。它对偶氮染料和三苯甲烷染料的混合物（两种类型染料的混合物）也显示出相当高的脱色效率，以及对混合染料植物毒性的强大解毒能力。粗漆酶对偶氮染料α - 萘酚橙（α - NO）和媒染剂蓝13（MB13）表现出强大的连续批次脱色能力。同样，它对三苯甲烷染料（如甲酚红、酸性绿50）实现了高连续批次脱色效率，同时在整个脱色过程中保持稳定的漆酶活性。粗漆酶在连续批次脱色过程中表现出优异的可重复使用性和可持续降解性能。粗漆酶的脱色可显著降低或完全消除单染料以及两种染料混合物（不同类型染料的两两混合物，共18种不同组合）的植物毒性。漆酶蛋白与结构多样的染料之间的分子对接结果进一步阐明了漆酶对不同结构染料催化能力变化的潜在原因和机制。总之，源自yang1的粗漆酶在高效降解和解毒不同结构类型的染料污染物方面具有巨大的应用价值和潜力。
