Manzano Vela Dennis Renato, Zabala Vizuete Rolando Fabian, Flores Mancheno Ana Carola, Salas Castelo Edison Marcelo
Facultad de Recursos Naturales, Escuela Superior Politécnica de Chimborazo (ESPOCH), Riobamba 060150, Ecuador.
Grupo de Investigación de Manejo y Aprovechamiento de los Recursos Renovables (GIMAR), Riobamba 060150, Ecuador.
Int J Mol Sci. 2025 Sep 4;26(17):8592. doi: 10.3390/ijms26178592.
The extraction of cellulose from underutilized forest residues can diversify bio-based material supply chains and reduce pressure on commercial pulps. In this study, cellulose was isolated from () pruning residues through an alkaline-acid-oxidative protocol, and its suitability for polymeric applications was evaluated. Two granulometric fractions (250 µm and 125 µm) were used; the yields were 4.73 ± 0.12 g and 3.62 ± 0.11 g per 50 g of biomass, equivalent to 90.5% and 92.8% recovery, respectively (fractional remains as bleached pulp after removal of non-cellulosic components). Fourier Transform Infrared spectroscopy (FTIR) showed the disappearance of lignin and hemicelluloses bands and a pronounced β-glucopyranosic signal at 894 cm, indicating high purity. Selective solubility in 17.5% NaOH classified the polymer as β-cellulose, suitable for wet spinning and film regeneration. Optical microscopy revealed smooth fibers of 25-50 µm length and 0.5-1 µm diameter, with aspect ratios ≥ 50, indicating favorable morphology for load transfer in composites. Statistical analysis (Shapiro-Wilk, F-test, and Student's -test) confirmed the significant influence of particle size on yield ( < 10). Overall, residues constitute a viable source of high-purity β-cellulose, whose molecular integrity and microstructure satisfy the requirements of sustainable polymeric manufacturing.
从不充分利用的森林残留物中提取纤维素可以使生物基材料供应链多样化，并减轻对商业纸浆的压力。在本研究中，通过碱-酸-氧化方案从（）修剪残留物中分离出纤维素，并评估了其在聚合物应用中的适用性。使用了两种粒度级分（250 µm和125 µm）；每50 g生物质的产量分别为4.73±0.12 g和3.62±0.11 g，分别相当于90.5%和92.8%的回收率（去除非纤维素成分后作为漂白浆的残留部分）。傅里叶变换红外光谱（FTIR）显示木质素和半纤维素谱带消失，在894 cm处有明显的β-吡喃葡萄糖信号，表明纯度高。在17.5% NaOH中的选择性溶解性将该聚合物归类为β-纤维素，适用于湿法纺丝和薄膜再生。光学显微镜显示长度为25-50 µm且直径为0.5-1 µm的光滑纤维，长径比≥50，表明在复合材料中有利于载荷传递的形态。统计分析（夏皮罗-威尔克检验、F检验和学生t检验）证实了粒径对产量有显著影响（<10）。总体而言，残留物构成了高纯度β-纤维素的可行来源，其分子完整性和微观结构满足可持续聚合物制造的要求。
