一种甾体α-酮醇氧化酶的增溶与分级沉淀
Solubilisation and fractional precipitation of a steroid alpha-ketol oxidase.
作者信息
Senciall I R, Rahal S, Sethumadhavan K
出版信息
J Steroid Biochem. 1985 Dec;23(6A):1083-5. doi: 10.1016/0022-4731(85)90072-x.
PMID:4094415
Abstract
An alpha-ketol oxidase that converts deoxycorticosterone to pregnenoic acid has been solubilised and fractionally precipitated with polyethylene glycol from rabbit liver microsomes. Maximal activity was obtained with the 12-14% fraction. Activity was linear with cytochrome P-450 concentrations up to 0.2 nmol and was inhibited with carbon monoxide.
摘要
一种能将脱氧皮质酮转化为孕烯酸的α-酮醇氧化酶已从兔肝微粒体中溶解出来，并用聚乙二醇进行分级沉淀。在12% - 14%的分级部分中获得了最大活性。活性在细胞色素P - 450浓度高达0.2 nmol时呈线性关系，且被一氧化碳抑制。
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