Kondashevskaya M V, Manukhina E B, Aleksankina V V, Artemyeva K A, Kaktursky L V
Avtsyn Institute of Human Morphology, Petrovsky National Research Center of Surgery, Moscow, Russia.
Institute of General Pathology and Pathophysiology, Moscow, Russia.
Dokl Biol Sci. 2025 Sep 14. doi: 10.1134/S0012496625600125.
Four groups of old Wistar rats were used: anesthesia only (An), noncardiac surgery (NS), stress exposure for 5 days followed by NS (SNS), and an intact control. Increases in proinflammatory cytokines and C-reactive protein were not observed within 10 days after surgery, suggesting no inflammation. Stress was evident from the highest corticosterone level in the SNS rats. Myocardial injury after NS (MINS) occurred in the SNS rats as judged from structural and metabolic disorders and an increase in the blood concentration of High-sensitive cardiac troponin I (hs-cTnI). Although ischemic and hypoxic zones in the myocardium were detected in the An and NS rats, the rats did not develop MINS, displaying neither oxidant-antioxidant imbalance nor, most importantly, an increase in hs-cTnI. The study showed for the first time that "simple" aseptic noncardiac surgery in combination with preoperative stress can induce MINS in healthy old Wistar rats. A decrease in superoxide dismutase was proposed as an additional biomarker of MINS.
使用了四组老年Wistar大鼠：仅麻醉组（An）、非心脏手术组（NS）、应激暴露5天后进行非心脏手术组（SNS）以及完整对照组。术后10天内未观察到促炎细胞因子和C反应蛋白增加，表明无炎症反应。从SNS组大鼠最高的皮质酮水平可明显看出存在应激。根据结构和代谢紊乱以及高敏心肌肌钙蛋白I（hs-cTnI）血浓度升高判断，SNS组大鼠在非心脏手术后发生了心肌损伤（MINS）。尽管在An组和NS组大鼠中检测到心肌存在缺血和缺氧区域，但这些大鼠并未发生MINS，既未表现出氧化-抗氧化失衡，最重要的是，hs-cTnI也未升高。该研究首次表明，“简单”的无菌非心脏手术与术前应激相结合可在健康老年Wistar大鼠中诱发MINS。超氧化物歧化酶的降低被认为是MINS的另一个生物标志物。
