Cheng Yuan-Cheng, Yang Yi-Ju, Chen Yi-Huey
Department of Life Science, National Taiwan Normal University, Taipei, Taiwan Department of Life Science, National Taiwan Normal University Taipei Taiwan.
Department of Natural Resources and Environmental Studies, National Dong-Hwa University, Hualien, Taiwan Department of Natural Resources and Environmental Studies, National Dong-Hwa University Hualien Taiwan.
Biodivers Data J. 2025 Sep 4;13:e160332. doi: 10.3897/BDJ.13.e160332. eCollection 2025.
is an alien species first recorded in 2006 in Taiwan. The expanding population of poses potential threats to the native frog species, especially to the closely-related species . To detect genetic diversity and analyse population structures of both native and alien species in Taiwan, this study aimed to isolate microsatellite markers in and test their cross-species amplification in the alien species . We successfully amplified and characterised 10 polymorphic microsatellite loci in . The number of alleles per locus ranged from 2 to 19 and no locus showed evidence of a null allele. The observed and expected heterozygosity ranged from 0.200 to 1.000 and 0.180 to 0.916, respectively and each locus was detected in Hardy-Weinberg equilibrium. Cross-amplification in was successfully performed in seven out of 10 loci. Amongst these seven loci, five exhibited polymorphism and two showed no variation. These microsatellite markers will be helpful for further population genetics research on species in Taiwan.
是2006年首次在台湾被记录的外来物种。其不断增长的种群数量对本地蛙类物种构成了潜在威胁，尤其是对亲缘关系密切的物种。为了检测台湾本地和外来物种的遗传多样性并分析其种群结构，本研究旨在分离该物种的微卫星标记，并测试它们在外来物种中的跨物种扩增情况。我们成功地扩增并鉴定了该物种的10个多态性微卫星位点。每个位点的等位基因数量从2到19不等，且没有位点显示出无效等位基因的证据。观察到的杂合度和预期杂合度分别在0.200到1.000和0.180到0.916之间，并且每个位点都处于哈迪-温伯格平衡状态。10个位点中有7个成功地在外来物种中进行了跨物种扩增。在这7个位点中，5个表现出多态性，2个没有变异。这些微卫星标记将有助于进一步开展台湾该物种的种群遗传学研究。
